2型糖尿病合并皮质性白内障患者房水中Txnip、Trx的定量检测及相关性分析  

Quantitative detection and correlation analysis of Txnip,Trx in aqueous humor of cortical cataract patients with type 2 diabetes

作　　者：姜凌峰[1] 石栋[1] 阎启昌[1] 

机构地区：[1]中国医科大学附属第四医院眼科中国医科大学附属眼科医院辽宁省晶状体学重点实验室,辽宁省沈阳市110005

出　　处：《眼科新进展》2017年第8期736-738,742,共4页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助项目(编号:81600777)~~

摘　　要：目的探讨2型糖尿病合并皮质性白内障的患者房水中硫氧还蛋白(thioredoxin,Trx)、硫氧还蛋白互作蛋白(thioredoxin interacting protein,Txnip)的含量以及与皮质性白内障程度的相关性。方法采用酶联免疫吸附实验(ELISA法)测定60例(60眼)明确诊断2型糖尿病合并皮质性白内障患者的房水中Trx及Txnip的含量,其中按照患者糖化血红蛋白(hemoglobin A1c,HbA1c)值将患者分组:A组(对照组,20例)HbA1c<5.5%;B组(20例)5.5%≤HbA1c<6.5%和C组(20例)HbA1c≥6.5%。结果与A组相比,B组患者房水中Txnip浓度增高,而C组浓度最高,差异均有统计学意义(均为P<0.05);与A组相比,B组Trx的浓度升高,而C组Trx的浓度最低,差异均有统计学意义(均为P<0.05)。A组中Trx与Txnip的浓度呈正相关(r=0.810,P=0.000);在C组中,Trx与Txnip的浓度呈负相关(r=-0.809,P=0.000),与白内障程度呈负相关(r=-0.727,P=0.001),其余均不相关(均为P>0.05)。Logistic回归分析显示,Txnip和HbA1c是白内障发病的危险因素。结论Trx、Txnip可能参与了糖尿病患者中白内障的发生发展。Objective To investigate the level and correlation between Thioredoxin （Trx） and Thioredoxin interacting protein （Txnip） in aqueous humor of cortical cataract patients with type 2 diabetes.Methods The Txnip and Trx levels were determined by Elisa in aqueous humor of 60 cataract patients with type 2 diabetes,which were divided into group A （control group,HbA1c〈5.5%）,group B （5.5%≤HbA1c〈6.5%） and group C （HbA1c≥6.5%）,and each group contains 20 patients.Results The level of Txnip in group B was higher than that in group A,while that in group C was the highest,there were statistical differences （all P〈0.05）.Compared with group A,the level of Trx in group B was increased,while that in group C was the lowest,there were statistical differences （all P〈0.05）.The level of Trx was positive correlated with Txnip in group A （r=0.810,P=0.000）,but negative correlated with Txnip in group C （r=-0.809,P=0.000） and degree of cataract in group C（r=-0.727,P=0.001）.Logistic regression analysis revealed that Txnip and HbA1c were risk factors of cortical cataract.Conclusion Trx and Txnip may play an important role in the development of diabetic cataract.

关 键 词：糖尿病性白内障 硫氧还蛋白 硫氧还蛋白互作蛋白 氧化应激 

分 类 号：R776.1[医药卫生—眼科]