原发性先天性青光眼(PCG)患儿与正常婴幼儿血浆中微小核糖核酸(miRNA或miR)-29b、miR-24、miR-200c的表达差异及临床意义  

Expression differences and clinical significance of miR-29b,miR-24 and miR-200c in plasma of infants with PCG and normal

作　　者：郑玲[1] 冯光强 

机构地区：[1]揭阳市人民医院眼科,广东省揭阳市522000 [2]广州市妇女儿童医疗中心眼科,广东省广州市510623

出　　处：《眼科新进展》2017年第8期755-758,共4页Recent Advances in Ophthalmology

基　　金：广东省卫生厅科技项目(编号:A2014567)~~

摘　　要：目的探讨原发性先天性青光眼(primary congenital glaucoma,PCG)患儿与正常婴幼儿血浆中微小核糖核酸(miRNA或miR)-29b、miR-24、miR-200c的表达差异及临床意义。方法收集2015年至2017年散发的PCG患儿16例血浆标本作为PCG组,另选年龄相近的正常婴幼儿共49例血浆标本作为正常人组。应用实时荧光定量PCR检测两组受试者血浆中miR-29b、miR-24、miR-200c的表达量,分析各自表达差异与病变严重程度的关系,并运用ROC曲线评价其诊断价值。结果PCG组受试者血浆中miR-29b、miR-24、miR-200c相对表达量(0.31±0.19、0.17±0.16、0.55±0.18)明显低于正常人组(1.18±0.52、2.86±2.65、1.62±0.76),差异均有统计学意义(均为P<0.05);血浆中miR-24、miR-29b的表达与PCG病变严重程度有关,病变越严重,其表达量越低;ROC曲线表明miR-24和miR-29b对PCG疾病的诊断价值高于miR-200c。结论血浆中游离的miRNA可作为辅助诊断PCG一种新的血浆标志物。Objective To identify the expression differences of mircoRNA-29 b（miR-29b）,microRNA？24（miR-24） and microRNA-200 c（miR-200c） in plasma of infants with primary congenital glaucoma （PCG） and normal,and analyze its clinical significance.Methods The expression quantity of microRNAs （miR-29b,miR-24,miR-200c） in plasma of PCG group （16 cases） and normal control group （49 cases） were detected by RT-PCR,and the relationship between their expression differences and severity of disease were analyzed.The diagnostic value of miRNAs for PCG was evaluated by receiver-operating characteristic curve （ROC）.Results The expression quantity of miR-29b,miR-24,miR-200c in PCG group （0.31±0.19,0.17±0.16,0.55±0.18,respectively） were significantly lower than those in normal control group （1.18±0.52,2.86±2.65,1.62±0.76,respectively） （all P〈0.05）;The expression quantity of miR-24 and miR-29b in plasma was related to the severity of PCG,the more severe the disease,the lower the expression;ROC curve indicated that miR-24 and miR-29b had a higher diagnostic value for PCG disease than miR-200c.Conclusion Free miRNAs in plasma may be used as a new plasma markers for auxiliary diagnosis of PCG.

关 键 词：微小核糖核酸-29 b 微小核糖核酸-24 微小核糖核酸-200c 血浆 原发性先天性青光眼 

分 类 号：R775.2[医药卫生—眼科]