HAI-1与子宫内膜癌侵袭转移的相关性研究  

The Relationship between HAI-1 and the Invasion and Migration of Endometrial Cancer Cells

作　　者：金琼[1] 缪飞[1] 黄勉[1] 孙蓬明[2] JIN Qiong MIAO Feil HUANG Mian SUN Peng-min(Department of Gynecology and Obstetrics, The First Hospital of Fuzhou, Fuzhou 350009, China Department of Gynecology, Fujian Province Maternal and Child Health Hospital, Fuzhou 350001, China)

机构地区：[1]福州市第一医院妇产科,福建福州350009 [2]福建省妇幼保健院妇科,福建福州350001

出　　处：《中国医药指南》2017年第19期1-2,4,共3页Guide of China Medicine

基　　金：福州市科技计划项目(2015-S-140-4)

摘　　要：目的探讨HAI-1表达水平与子宫内膜癌细胞株侵袭迁移能力的相关性。方法体外培养人子宫内膜癌细胞株,采用Real-time q PCR技术检测HEC-1A、HEC-1B和RL952三株子宫子宫内膜癌细胞中HAI-1 m RNA表达水平,应用细胞划痕实验及穿膜小室实验,观察三株子宫内膜癌细胞体外的侵袭及迁移能力。结果 (1)HAI-1 m RNA在三株细胞中的相对表达量分别为(0.3042±0.0101)、(0.0032±0.0001)和(0.2580±0.0096),HAI-1蛋白在HEC-1A、HEC-1B和RL952中均呈阳性表达,但在HEC-1B中表达低于HEC-1A及RL952。(2)细胞划痕实验结果显示,HEC-1A、HEC-1B和RL952三组迁移距离分别为(173.30±7.33)μm、(437.00±8.72)μm和(135.30±6.89)μm。(3)侵袭实验结果显示,HEC-1A、HEC-1B和RL952三组穿膜细胞数分别为(52.67±2.73)、(117.30±3.48)和(62.00±3.06)。结论 HAI-1 m RNA表达水平与子宫内膜癌细胞侵袭迁移能力呈负相关。对HAI-1抑癌机制的进一步研究,将有望使HAI-1成为今后子宫内膜癌诊断及治疗中的新靶点。Objective To explore the relationship between HAI-1 and the invasion and migration of endometrial cancer cells. Methods Detect three endometrial cancer cells（HEC-1A, HEC-1B and RL952） m RNA expression level of HAI-1 by Q-RTPCR. Then, the ability of invasion and migration were compared between the three endometrial cancer cells using scratch assay and transwell chamber assay separately. Results（1）The relative expression of HAI-1 m RNA in three cells was（0.3042±0.0101）,（0.0032±0.0001）,（0.2580±0.0096）, HAI-1 protein was positive in HEC-1A, HEC-1B and RL952, but was lower than HEC-1A and RL952 in HEC-1B.（2）The scratch assay showed that the migration distance of HEC-1A, HEC-1B and RL952 were（173.30±7.33）μm,（437.00±8.72）μm and（135.30±6.89）μm.（3）The millicell chamber invasion assay showed that the ability of invasion were（52.67±2.73）,（117.30±3.48） and（62.00±3.06）. Conclusion The invasion and migration of endometrial cancer cells was negative related with the HAI-1 expression, Further study on the mechanism of HAI-1 tumor suppressor is expected to make HAI-1 is expected to be a new target in the diagnosis and treatment of endometrial carcinoma in the future.

关 键 词：HAI-1基因 子宫内膜癌 转移 侵袭 

分 类 号：R737.33[医药卫生—肿瘤]