溶菌酶结晶法提取及其酶活力的探讨  

Study on extraction and enzyme activity of lysozyme by salting out method

作　　者：黄赞良 谈海玉 邓彩思 张兆霞[1] 李泳[1] 

机构地区：[1]广东海洋大学,广东湛江524088

出　　处：《云南化工》2017年第5期83-86,共4页Yunnan Chemical Technology

基　　金：广东海洋大学团队项目(项目编号:C13435);大学生创新创业项目(项目编号:CXXL2016152)

摘　　要：以湖光岩鹌鹑蛋为原料,采用结晶法从蛋清中分离提取溶菌酶。探讨不同条件对溶菌酶收率及活性的影响,确定溶菌酶最佳的分离提取条件:盐析Na Cl质量分数为5.1%,pH=10.7,盐析时间为120h,收率达到0.378%,酶活力达到13700 U/mg。By salting out extraction of lysozyme from egg white in quail, the effects of different conditions on the extraction of lysozyme were investigated. The optimum extraction conditions were determined： NaCl concentration was 5.1%, salting out pH value was 10.7, Salting out time is 120h, Extraction yield reached 0.378%. The enzyme activity of lysozyme was determined to be 13700 U/rag by turhidimetry.

关 键 词：盐析法 溶菌酶 酶活力 

分 类 号：S188[农业科学—农业基础科学]