阿霉素干预对miR-106a在淋巴瘤Jurkat细胞中表达的影响  

The Effect of Adriamycin Intervention on the Expression of miR-106a in Lymphoma Jurkat Cells

作　　者：刁玉巧[1] 唐国英[1] 曲凡[1] 朱秀丽[1] 

机构地区：[1]河北医科大学第四医院儿科,石家庄050011

出　　处：《基因组学与应用生物学》2017年第7期2660-2664,共5页Genomics and Applied Biology

基　　金：河北医科大学第四医院资助

摘　　要：检测miR-106a在淋巴瘤Jurkat细胞中的表达及阿霉素对其表达的影响,探讨miR-106a在淋巴瘤发病中的作用机理。采用淋巴瘤Jurkat细胞,用不同浓度阿霉素处理,以正常人外周血单个核细胞作对照,应用实时定量PCR(Real-Time PCR)方法检测上述各组miR-106a中的表达情况;采用MTT方法检测Jurkat细胞增殖情况;采用流式细胞术(FCM)分析阿霉素干预后Jurkat细胞凋亡及周期变化。淋巴瘤Jurkat细胞中miR-106a表达水平明显高于正常对照组(t=10.27,p<0.01)。用不同浓度阿霉素作用于Jurkat细胞48 h、72 h后,miR-106a表达水平较对照组均下降,并随阿霉素浓度升高其相对表达量降低(48 h F=198.57,72 h F=495.43,p均<0.01);对Jurkat细胞的增殖抑制率(48 h F=65.54,72 h F=110.08,p均<0.01)和凋亡率均高于对照组(48 h F=1072.19,72 h F=3 271.66,p均<0.01)。miR-106a在淋巴瘤Jurkat细胞中的表达较正常人增高,阿霉素对其表达有抑制作用,miR-106a可能与淋巴瘤的发生发展有关。The aim of this study was to identify the expression of miR-106a in lymphoma Jurkat cell and the effect of adriamycin on it, and explore the mechanism of miR-106a in the pathogenesis of lymphoma. The lymphoma Jurkat cell was treated by adriamycin with different concentrations. The normal peripheral blood mononuclear cells were set as the control and the real time PCR was applied to detect the expression of miR-106a in the above groups. The proliferation of Jurkat cell was measured by MTT assay. The apoptosis and periodic change of Jurkat cells after the treatment ofadriamycin were analyzed by Flow cytometry （FCM）. The expression level of miR-106a in lymphoma Jurkat cell was obviously higher than that in normal control group （t= 10.27, p 〈0.01）. Compared with the control group, the expression level of miR-106a decreased after the Jurkat cell was treated by adriamycin with different concentrations for 48 h and 72 h, and the expression level decreased with the increase of the concentration ofadriamycin （48 h F=198.57, 72 h F=495.43,p〈0.01）. The proliferation inhibition rate （48 h F= 65.54, 72 h F=110.08,p〈0.01） and apoptosis rate （48 h F=1072.19, 72 h F=3 271.66,p〈0.01） on Jurkat cell were both higher than the control group. The expression level of miR-106a level in lymphoma Jurkat cell was higher than that in normal people, while adriamycin had inhibiting effect on it, therefore, miR-106a might be associated with the occurrence and development of lymphoma.

关 键 词：淋巴瘤 miR-106a JURKAT细胞 阿霉素 

分 类 号：R733.1[医药卫生—肿瘤]