小型猪动脉粥样硬化模型中饲料配方的探讨  

Discussion of Fodder Prescription of Atherosclerotic Animal Model in Minipig

作　　者：冯元洁 尹妮[2] 高玉竹[1] 周鹤[1] 王双[1] 张哲[2] 杨关林[1] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110847 [2]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《中华中医药学刊》2017年第8期2052-2055,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)项目(2013CB531704)

摘　　要：目的:探讨本实验中高脂饲料对血脂的影响及饲料组分的具体作用。方法:将55只巴马小型猪分为两组,正常组5只,手术组50只。正常组予基础饲料喂饲,手术组予高脂喂饲,实验周期为48周。手术组于第2周行冠状动脉机械损伤术,各组于第24、28、36、48周分别测定血脂水平变化。结果:因手术组分为模型组和药物干预组,故仅对正常组和模型组进行统计,以便观察高脂喂饲对血脂的影响。第24、28、36、48周时,模型组TC、TG、LDL-C、ox-LDL水平明显高于正常组,统计学差异显著(P<0.01);模型组HDL-C水平在第24周明显高于正常组,统计学差异显著(P<0.01);随后逐渐呈下降趋势但仍高于正常组,在28、36周时两组比较差异有统计学意义(P<0.01,P<0.05),48周时两组比较差异无统计学意义(P>0.05)。结论:本实验采用的高脂饲料能够在短时间内造成小型猪的高脂血症,促进冠状动脉粥样硬化的形成。Objective：To research the effect of high-fat diet on blood lipid in the experiment and the specific function of fodder components.Methods：Fifty-five pigs were randomly assigned to the blank group（n=5） and operation group（n=50）.They were fed with regular diet and high-fat diet respectively.At the 2 nd week,pigs of operation group were conducted with balloon abrasion of the coronary artery.At the 24 th,28 th,36 th and 48 th week,observe the blood lipid level of each group.Result：Only blank group and model group（one of the operation group） were counted in this article to easily observe the effect of high-fat diet on blood lipid.Because the other five groups of operation groups underwent drug intervention since the 24 th week.At the 24 th,28th,36 th and 48 th week,TC,TG,LDL-C and ox-LDL levels of model group were higher than those of the normal group with significantly difference（P〈0.01）.HDL-C level increased at first and then decreased.Two groups were significantly difference at the 24 th（P〈0.01）,28 th（P〈0.01） and 36 th（P〈0.05） week,and there was no statistical difference at the 48 th week.Conclusion：The high-fat diet in this experiment could cause hyperlipidemia of minipig within a short time to accelerate the development of coronary atherosclerosis.

关 键 词：动脉粥样硬化 动物模型 高脂饲料 

分 类 号：R-332[医药卫生]