高效液相色谱柱后衍生法测定归脾丸中的黄曲霉毒素  

Determination of Aflatoxins in Guipi Pills by HPLC Method with Post-column Derivatization

作　　者：栗建明[1] 顾利红[1] 隆颖[2] 

机构地区：[1]广州市药品检验所,广东广州510160 [2]广东省药品检验所,广东广州510180

出　　处：《今日药学》2017年第7期460-462,共3页Pharmacy Today

基　　金：广东省中医药管理局项目(20151048)

摘　　要：目的建立归脾丸中黄曲霉毒素G_2、G_1、B_2、B_1的残留量测定方法。方法样品经70%甲醇提取、免疫亲和柱净化后,用高效液相色谱-柱后光化学衍生-荧光检测器对归脾丸中4种黄曲霉毒素(G_2、G_1、B_2、B_1)的残留量进行分析测定。结果黄曲霉毒素G_2在1.232~24.64 pg范围内,黄曲霉毒素B_2在1.212~24.24 pg范围内,黄曲霉毒素G_1、B_1在4.148~82.96 pg范围内,线性关系良好,相关系数均r≥0.999 4。回收率在83.8%~101.4%之间。结论该方法快速简便、准确,可作为归脾丸中黄曲霉毒素残留量的测定方法。OBJECTIVE To establish a method for the residual determination of aflaroxin G_2,G_1,B_2,B_1 in Guipi Pills.METHODS After being extracted by 70% methanol and purified by immunoaffinity columns,aflatoxins G_2,G_1,B_2,B_1 were analyzed by HPLC with post-column photochemistry derivatization and fluorescence detector. RESULTS Aflatoxin G_2 showed a good linear relationship at a range of 1.232-24.64 pg,and aflatoxin B_2 at a range of 1.212-24.24 pg,Aflatoxin G_1,B_1 at a range of 4.148-82.96 pg,r≥0.999 4. The recovery was between 83. 8%-101. 4%. CONCLUSION The method is simple,accurate and can be used to determine aflatoxins G_2,G_1,B_2,B_1 in Guipi Pills.

关 键 词：黄曲霉毒素 归脾丸 高效液相色谱法 柱后光化学衍生法 

分 类 号：R284.1[医药卫生—中药学]