骶神经电刺激对慢性传输型便秘大鼠结肠中Cajal间质细胞c-kit表达的影响  

Sacral nerve stimulation for existence of c-kit positive cells of interstitial cells of Cajal in the subserosal layer of the STC rat

作　　者：柳凯伦 王志民[2] 孔凡娟[3] 陈璐[1] 

机构地区：[1]潍坊医学院,山东潍坊261053 [2]山东省千佛山医院肛肠科,山东济南250014 [3]山东中医药大学,山东济南250355

出　　处：《中国现代普通外科进展》2017年第6期431-434,439,共5页Chinese Journal of Current Advances in General Surgery

基　　金：山东省自然科学基金项目(ZR2014HL017)

摘　　要：目的:通过测量c-kit的量研究骶神经电刺激(SNS)对慢性传输型便秘(STC)大鼠结肠组织中Cajal间质细胞(ICC)数量和功能的影响。方法:将30只Wistar大鼠随机分为STC组、SNS治疗组、正常组3组,每组10只。STC组和SNS治疗组采用复方地芬诺酯灌胃,制造STC大鼠模型。骶神经电刺激治疗STC组大鼠。治疗结束后,脱颈处死各组大鼠,采集标本后通过免疫组化、Western blot和实时定量RT-PCR来检测各组大鼠结肠表达c-kit的差异。结果:SNS治疗之前,STC组及SNS治疗组大鼠的体重和粪便干重均低于正常组。SNS治疗后,SNS治疗组大鼠的体重及粪便干重明显增加。电刺激治疗后,SNS治疗组c-kit明显多于STC组,差异有统计学意义(P<0.05)。结论:SNS可促进ICC中c-kit的表达,提高ICC的数量和功能,改善STC大鼠的症状,为便秘治疗提供一种新方法。Objective： To evaluate the effect of sacral nerve stimulation （SNS） for STC by re ceptor tyrosine kinase c-kit. Methods： Wistar rats were fed with diphenoxylate to make slow transit constipation（STC） rat model. Sacral nerve stimutation（SNS） for the SNS Rat Group. The studied animals were allocated into three experimental groups： STC Rat Group; SNS Rat Group; Normal Rat Group; Every group included 10 rats. The c-kit of ICC in the subserosal layer of rats were analyzed by immunohistochemistry, western blot and RT-PCR. Results： The morphological characteristics of STC Rat Group were not comparable to those of the multipolar c-Kit positive ICC seen in the subserosa of colon of normal rat. In the colon of rat, c-kit protein and c-kit gene in SNS rat group and Normal rat group was significantly higher than STC rat group detecting by western blot. Statistical differences between STC Group and SNS Rat Group were found （P〈0.05）; statistical differences between STC Group and Normal Rat Group were also found （P〈0.05）. There was not statistical differences between SNS rat group and Normal Rat Group. Conclusion： SNS has effective treatment for the STC rat.

关 键 词：慢性传输型便秘 骶神经电刺激 CAJAL间质细胞 C-KIT WIS tar大鼠 

分 类 号：R574.62[医药卫生—消化系统]