ki-67与survivin的表达与大肠腺瘤癌变的相关性分析  

Correlation of expression of ki-67 and survivin with the canceration of colorectal adenoma

作　　者：常圆[1] 关景明[2] CHANG Yuan GUAN Jingming(Department of Gastoenterology and Hepatology ,the Fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongj iang 150000, China Department of Gastoenterolog y and Hepatologym,the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086 ,China)

机构地区：[1]哈尔滨医科大学附属第四医院消化内科,哈尔滨150000 [2]哈尔滨医科大学附属第二医院消化内科,哈尔滨150086

出　　处：《检验医学与临床》2017年第15期2222-2224,共3页Laboratory Medicine and Clinic

摘　　要：目的检测正常大肠黏膜、大肠腺瘤、伴鸡皮样黏膜(CSM)改变的大肠腺瘤和腺瘤癌变组织中代表细胞增殖的标志物ki-67和凋亡相关因子survivin的表达情况,探讨其与大肠腺瘤癌变的关系。方法采用免疫组织化学法检测正常大肠黏膜、伴CSM改变的大肠腺瘤、无CSM改变的大肠腺瘤和大肠腺瘤癌变标本中代表细胞增殖的标志物ki-67和细胞凋亡相关因子survivin的表达,每种标本各18例。结果免疫组织化学结果提示,ki-67阳性反应产物位于细胞核内,呈棕褐色,细胞质一般不着色。survivin主要表达于细胞质,其蛋白阳性反应为淡黄到棕黄色颗粒,在正常黏膜中不表达。在大肠腺瘤癌变组织、伴CSM改变的大肠腺瘤、大肠腺瘤及正常大肠黏膜中ki-67的阳性表达率分别是77.8%、83.3%、33.3%、5.6%。在大肠腺瘤癌变组织、伴CSM改变的大肠腺瘤、大肠腺瘤及正常大肠黏膜中survivin的阳性表达率分别是72.2%、77.8%、33.3%、0.0%。与正常大肠黏膜组织相比,其他3中黏膜组织的ki-67和survivin阳性率均升高,差异有统计学意义(P<0.05);伴CSM改变的大肠腺瘤及腺瘤癌变组织ki-67和survivin阳性率高于大肠腺瘤,差异有统计学意义(P<0.05)。结论伴CSM改变的大肠腺瘤ki-67与survivin的表达升高,存在活跃的细胞增殖,同时细胞凋亡受抑制,这种生物学特性的改变使伴CSM改变的大肠腺瘤癌变的概率增加。Objective To examine the expression of ki-67 and survivin in the normal colorectal mucosa,colorectal adenoma,adenoma surrounding with chicken skin mucosa（CSM） and colorectal adenoma canceration. And to explore the relationship between the expression of ki-67 and survivin and canceration of colorectal adenoma. Methods Immunohistochemical staining was used to exam- ine the expression ki-67 and survivinin in the normal colorectal mucosa, colorectal adenoma, adenoma surrounding with CSM and canceration of colorectal adenoma. Every type of tissue had 18 cases. Results The browned positive products of ki-67 was located in the nucleus, and the cytoplasm was not staining. Survivin was mainly expressed in cytoplasm,and its positive products were yellow- ish to brownish yellow granules,and it was not expressed in normal mucosa. The positive expression rates of ki-67 in the cancera- tion of colorectal adenoma, adenoma surrounding with CSM, colorectal adenoma and normal colorectal mucosa were 77.8 %, 83.3 %, 33.3% and 5.6%,respectively. And the positive expression rates of ki-67 in the canceration of colorectal adenoma, adenoma sur- rounding with CSM, colorectal adenoma and normal colorectal mucosa were 72.2 %, 77.8%,33.3 % and 0.0 %. The positive expres- sion rates of ki-67 and survivin in the other three types of tissues were significantly higher than that in the normal colorectal mucosa （P〈0.05）. And the positive expression rates of ki-67 and survivin in canceration of coIorectal adenoma and adenoma were signifi- cantly higher than that in the colorectal adenoma（P〈0.05）. Conclusion The expression of ki-67 and survivin in the colorectat ade- noma with CSM has increased,as well as the presence of active cell proliferation and inhibition of cell apoptosis. Theses changes of the biological characteristics make the probability of eolorectal adenoma with CSM increased.

关 键 词：大肠腺瘤 鸡皮样黏膜 细胞增殖 

分 类 号：R735.7[医药卫生—肿瘤]