香乳菇多糖对Hela细胞凋亡及对RAW264.7细胞免疫活性的影响  

Inhibitory effect on Hela cells and immune effects on RAW264.7 cells of a polysaccharide from Lactarius camphoratum(Bull.)Fr

作　　者：赵大群 丁祥[1,2] 刘露[1,2] 钱叶[1,2] 许婷[1,2] 苟凡铖 宋波[1,2] 侯万儒[1,2] 侯怡铃[1,2] 

机构地区：[1]西华师范大学生命科学学院,四川南充637009 [2]西南野生动植物资源保护教育部重点实验室,四川南充637009

出　　处：《食品与机械》2017年第6期1-6,共6页Food and Machinery

基　　金：国家自然科学基金项目(编号:31400016);四川省生态安全与保护重点实验室开放基金资助项目(编号:ESP1408);四川省教育厅重大培育项目(编号:16CZ0018);南充市科技局项目(编号:16YFZJ0043)

摘　　要：为研究香乳菇多糖(LC-1)的抗肿瘤和免疫调控活性,将人的宫颈癌Hela细胞和小鼠巨噬细胞RAW264.7体外培养,分别通过CCK-8法、流式细胞术和ELISA技术检测不同浓度LC-1对Hela细胞增殖和凋亡周期的影响,对RAW264.7细胞增殖、吞噬的影响,及对RAW264.7细胞分泌NO、IL-6和TNF-α的影响。结果表明:香乳菇多糖对体外培养的Hela细胞有明显的抑制作用,且影响Hela细胞的形态及凋亡周期,当LC-1浓度为10μg/mL时,Sub峰含量可达19.4%;同时,LC-1能调控巨噬细胞的免疫活性,当LC-1浓度为10μg/mL时,巨噬细胞增值率和吞噬活性分别为67.48%和87.09%,亦能促进巨噬细胞从G0/G1期向G2期和S期转化,同时刺激巨噬细胞产生IL-6和TNF-α,且呈现出明显的剂量依赖性,但对NO生成没有明显的促进作用。综上,在体外试验中,香乳菇多糖LC-1能抑制宫颈癌Hela细胞生长,促进RAW264.7细胞增殖和吞噬活性,刺激巨噬细胞产生免疫因子。The antitumor and immunoregulatory activities of polysaccharide LC-1fromLactarius camphoratum（Bull.）Fr.were studied by using the human cervical cancer Hela cells and the mouse macrophage RAW264.7cells.The two kinds of cells were cultured in vitro,and the effects of different concentrations of LC-1on the proliferation and cell cycle of Hela cells and the proliferation and phagocytosis of RAW264.7cells were investigated.Moreover,the secretion of NO,IL-6and TNF-alpha of RAW264.7cells were tested using CCK-8method,flow cytometry and ELISA technique,respectively.The results showed that polysaccharide LC-1 from L.camphoratum（Bull.）Fr.has obvious inhibitory effect on Hela cells in vitro,and could influence the apoptosis cell cycle of Hela cells.When the concentration of LC-1 was 10μg/mL,the content of Sub peaks was19.4%.It was further found that polysaccharide LC-1could regulate the immune activity of macrophage.When the concentration of LC-1was 10μg/mL,the phagocytic activity and proliferation rate of macrophage were 67.48% and 87.09%,respectively.LC-1could also promote the transformation of macrophages from G0/G1 phase to G2 phase and S phase and stimulate macrophages to produce IL-6and TNF-α,showing a dose-dependent.However,no obvious effect on the production of NO was observed.In conclusion,in vitro,polysaccharide LC-1from L.camphoratum（Bull.）Fr.could inhibit the growth of cervical carcinoma Hela cells and promote the proliferation and phagocytosis of macrophage,as well as stimulate macrophages to produce immune factors.

关 键 词：香乳菇多糖 HELA细胞 凋亡 巨噬细胞RAW264.7 增殖作用 吞噬作用 

分 类 号：R73-3[医药卫生—肿瘤]