结直肠癌放疗患者粪便具核梭杆菌变化研究  

Changes of fusobacterium nucleatum in colorectal cancer patients after radiotherapy

作　　者：连建春 刘蓓蓓[1] 张媛媛[1] 陆俊忠[1] 张迎梅[1] 姜玉章[1] 张小云[1] 

机构地区：[1]南京医科大学附属淮安第一医院检验科,江苏淮安223300

出　　处：《国际检验医学杂志》2017年第15期2051-2053,共3页International Journal of Laboratory Medicine

基　　金：南京医科大学科技发展基金资助项目(2016NJMU144)

摘　　要：目的研究具核梭杆菌在结直肠癌放疗患者中的感染率,以及放疗前后、肿瘤复发后的体内变化情况。方法经肠镜证实无肠道疾病体检者20例纳入对照组,收集粪便标本。40例结直肠癌患者纳入研究组,术前3d、术后5d、术后放疗7d、放疗30d均收集粪便标本,随访1年,复发者也收集粪便标本。采用过滤收集菌液沉淀,进行实时荧光定量PCR方法检测粪便标本中的核梭杆菌基因相对表达量。结果 40例结直肠癌患者中粪便具核梭杆菌的阳性率为30.0%,对照组阳性率为5.0%。12例结直肠癌阳性患者在术前3d、术后5d、术后放疗7d、放疗30d基因相对表达量分别为8.50±0.45、1.20±0.22、5.2±0.34、0.20±0.15。12例Fn阳性患者在术后5d的Fn基因相对表达量明显高于术前3d,差异有统计学意义(t=10.419,P=0.001),放疗7d、放疗30d的Fn基因相对表达量明显低于术前3d,差异均有统计学意义(t=12.728,P=0.001;t=25.889,P=0.001)。6例患者在手术后1年内复发,Fn基因相对表达量为7.20±0.56,复发时的Fn基因相对表达量较放疗后7d明显升高,差异有统计学意义(t=14.427,P=0.001)。结论具核梭杆菌感染可能是结直肠癌的高危因素,其基因相对表达量的升高可能作为疾病复发的预警指标。Objective To study the infection rate of fusobacterium nucleatum cancer re appeared in patients with colorectal cancer before and after radiotherapy,and the changes after cancer recarrence.Methods A total of 20 persons receiving physical examination were recruited in the control group and collected the stool specimens,and 40 colorectal cancer patients were selected in the study group.All of the subjects in the study group were collected stool specimens before operation 3days and after operation 5day,after radiation therapy 7days and 30 days.The patients were followed-up 1year.The bacterial fluid was collected by filtration,and real-time fluorescence quantitative PCR was used to detect the expression of fusobacterium nucleatum gene in feces.Results The positive rate of fecal fusobacterium fusiformis was 30%in the study group and 5%in the control group.The gene relative expression of 12 colorectal cancer patients before operation 3days and after operation 5days,after radiation therapy 7days and 30 days were 5.20±0.34,8.50±0.45,1.20±0.22,0.20±0.15.The fusobacterium nucleatum gene expression of 12 patients with positive fusobacterium after operation 5days was significantly increased compared with that before operation 3days(t=10.419,P=0.001),which after radiation therapy 7days and 30 days was significant lower than that before operation 3days(t=12.728,P=0.001;t=25.889,P=0.001).Six patients recurred among 1year,the fusobacterium nucleatum gene expression was 7.2±0.56,which was significant higher than that after radiation therapy 7days.Conclusion The infection of fusobacterium nucleatum might be a risk factor for colorectal cancer,and the gene relative expression might be an early warning indicator of recurrence.

关 键 词：结直肠癌 具核梭杆菌 放疗 实时荧光定量PCR 

分 类 号：R730.43[医药卫生—肿瘤] R735.34[医药卫生—临床医学]