长链非编码RNA CCAL在肝癌中的表达及其对预后的影响  

LncRNA-CCAL is highly expressed in hepatocellular carcinoma and predicts poor prognosis

作　　者：王雅玮[1] 杨野[1] 刘宇[1] 王欣[1] 李铁民[1] 石悦[1] 

机构地区：[1]中国医科大学附属第一医院老年外科,辽宁沈阳110001

出　　处：《现代肿瘤医学》2017年第17期2774-2778,共5页Journal of Modern Oncology

摘　　要：目的:检测LncRNA-CCAL在肝细胞癌(HCC)中的表达水平,并分析其与肝癌患者临床病理因素及预后的关系。方法:运用RT-PCR技术检测37例肝癌新鲜组织中CCAL的表达水平;运用原位杂交技术(ISH)检测120例肝癌患者组织切片中CCAL的表达水平,并运用卡方检验、COX回归模型、Kaplan-Meier生存曲线分析CCAL表达水平与肝癌患者临床病理因素及预后的关系。结果:37例肝癌新鲜组织标本中29例高表达,且癌组织中平均表达水平明显高于癌旁组织;120例肝癌患者组织切片中85例高表达,其表达水平与肝癌TMN分期和是否转移相关,而且CCAL较高表达的患者预后也相对较差,总生存率明显低于CCAL低表达的患者。结论:CCAL在肝细胞癌中高表达,是肝癌患者预后的独立影响因子。Objective：To test the expression of LncRNA - CCAL in hepatocellular cancer （ HCC） and study its influence on HCC prognosis. Methods：Use RT - PCR and in situ hybridization（ ISH） were used as test the expres-sion level of CCAL in HCC. Chi - square test,COX regression,Kaplan - Meier survival curve were used as analyze the relationship between CCAL and prognosis. Results ： RT - PCR and ISH both showed CCAL was overexpressed in HCC;CCAL expression was related with tumor metastasis,TNM stage and poor prognosis in HCC. Conclusion： Ln-cRNA -CCAL overexpression may be useful as an independent prognostic biomarker for HCC prognosis.

关 键 词：肝细胞癌 RT-PCR 原位杂交 CCAL 预后 

分 类 号：R735.7[医药卫生—肿瘤]