RYBP介导白血病细胞凋亡的靶基因预测  

Prediction of the target genes mediated by RYBP in the leukemic cell apoptosis

作　　者：李秋兰[1] 王顺清[1] 张玉平[1] 陈晓燕[1] 谢健晋[1] 吴亮亮[1] 

机构地区：[1]广州医科大学附属广州市第一人民医院血液内科,广州市510180

出　　处：《实用医学杂志》2017年第16期2620-2625,共6页The Journal of Practical Medicine

基　　金：广东省自然科学基金(编号:2014A030313676);中华医学会临床医学科研专项资金(编号:16010050621);广州市科技计划项目(编号:201508020254)

摘　　要：目的探讨RYBP介导白血病细胞凋亡的靶基因。方法利用慢病毒介导的RNA干扰技术,建立多批次RYBP基因稳定沉默的HL60细胞株及空载体对照组(NC);通过二代测序技术对不同批次干扰的上述3对细胞进行mRNA测序及miRNA测序,利用KEGG等生物信息学分析方法 ,预测靶基因。结果成功构建RYBP稳定沉默的HL60细胞株。二代测序筛选出明显差异的mRNA及miRNA(P<0.05)。Pathway显著性富集分析发现表达下调的基因与氨基酸合成,癌症转录调节紊乱,氨基酸代谢等相关的KEGG pathway有关,详见后述;沉默RYBP后,SPI1与hsa-miR-214-3p均表达降低(P<0.05)。结论RYBP介导白血病细胞凋亡的靶基因可能为:BBC3、BAI1、SESN2、CCNG2、JAK3、STAT4、SPI1、BCL6、CD11b和hsa-miR-214-3p。Objective To investigate the target genes in RYBP-mediated leukemia cell apoptosis by highflux sequencing.Methods The HL-60 cell line with knockdown of RYBP was set up.MRNA and microRNA sequencing was conducted by the next-generation sequencing technology.Result MRNAs and miRNAs were dysregulated in HL-60 cell line with knockdown of RYBP.The results of KEGG analysis indicated that the down-regulated genes were associated with cancer transcription regulation and amino acid metabolism.SPI1 and miR-214-3p were shown downregulated after knockdown of RYBP.Conclusion The target genes in RYBP-mediated leukemia cell apoptosis include BBC3,BAI1,SESN2,CCNG2,JAK3,STAT4,SPI1,BCL6,CD11b and hsa-miR-214-3p.

关 键 词：RYBP 白血病 二代测序 靶基因 

分 类 号：R733.7[医药卫生—肿瘤]