超声微泡介导质粒转染对人肝癌HepG2细胞迁移、侵袭及克隆能力的影响  

Effect of ultrasound microbubble mediated miRNA delivery on the ability of migration,invasion and cloning of human hepatoma HepG2 cells

作　　者：朱芳方 陈海清[2] 赖己创 陈佳琳[1] 过新民[1] 

机构地区：[1]暨南大学医学院附属广州市红会医院超声诊断科,广州市510220 [2]广东药学院附属广钢医院超声诊断科,广州市510380

出　　处：《实用医学杂志》2017年第16期2629-2633,共5页The Journal of Practical Medicine

基　　金：广东省科技计划项目立项(编号:2016A020215015)

摘　　要：目的探讨超声微泡造影剂介导miRNA质粒转染人肝癌HepG2细胞后迁移、侵袭及克隆能力的改变。方法在前期实验所筛选的最佳超声微泡转染条件下,转染目的基因反义miR-21/221、miR-199a进入人肝癌HepG2细胞,划痕实验、Transwell实验检测细胞迁移、侵袭能力,软琼脂克隆形成实验检测细胞克隆能力。结果转染目的质粒后,细胞的迁移、侵袭能力及克隆能力与对照组比较均显著受到抑制(P<0.05),其中以miR-199a质粒组的抑制效果最佳(相对细胞迁移率31.05%;平均视野侵袭细胞数38.67±4.51;克隆数105.67±5.86),与其它目的质粒组比较,差异均有统计学意义(P<0.05)。结论通过分析miRNAs对人肝癌HepG2细胞部分细胞功能的影响,为肝癌的基因治疗提供新的靶点及思路。Objective gration,invasion and cloning ability of human hepatoma HepG2 cells. Methods The migration,invasion and col-ony forming ability of HepG2 cells were measured after transfection with antisense miRNA-21/221 and mi RNA-199 a mimic via the optimal ultrasound microbubble transfection method. Results Compared with the controlgroup,the migration,invasion and cloning ability of cells were significantly inhibited after transfection withmi RNA mimics（P〈0.05,respectively）,especially for miR-199a（relative cell migration rate was 31.05%,thenumber of invasive cells were 38.67 ± 4.51 and the number of clones were 105.67 ± 5.86）. Conclusion The pres-ent study may provide new ideas and clues for gene therapy and prognosis of hepatocell ular carcinoma through ana-lyzing the effect of miRNAs on the biological characteristics of human hepatoma HepG2 cells.

关 键 词：超声微泡造影剂 miR-21 MIR-221 miR-199a 基因转染 人肝癌HEPG2细胞 

分 类 号：R735.7[医药卫生—肿瘤]