昆明小鼠与BALB/c小鼠建立变应性鼻炎模型的比较  

The comparation of establishing the allergic rhinitis model between Kunming mice and BALB/c mice

作　　者：高娴[1,2] 李娜[1] 张继生[1] 何凡[3] 

机构地区：[1]青岛市市立医院耳鼻咽喉头颈外科 [2]青岛大学附属医院耳鼻咽喉头颈外科,266003 [3]浙江省疾病预防控制中心,杭州310051

出　　处：《中华耳鼻咽喉头颈外科杂志》2017年第7期501-505,共5页Chinese Journal of Otorhinolaryngology Head and Neck Surgery

摘　　要：目的 探讨昆明小鼠建立变应性鼻炎（allergic rhinitis,AR）模型的可行性.方法 2015年9月至2016年1月期间,采用卵清蛋白（OVA）致敏并激发昆明小鼠（n=20）及BALB/c小鼠（n=20）,建立AR模型,待建模过程结束后,采用症状评分量表（症状总分＞5分为建模成功）评价两种小鼠的AR症状;再通过昆明小鼠模型组及空白对照组血清OVA特异性IgE （OVA-sIgE）及白细胞介素4（IL-4）的水平,评估昆明小鼠模型的血清学指标;将建模成功的昆明小鼠及BALB/c小鼠的各项指标进行比较,分析两种小鼠动物模型的差异.采用SPSS 22.0统计软件进行统计学分析.结果 实验过程中,BALB/c小鼠模型组死亡2只,昆明小鼠模型组无死亡,且建模后体重有一定的增长.昆明小鼠模型组的临床症状评分、血清OVA-sIgE水平、血清IL-4水平均显著高于空白对照组,差异具有统计学意义[（6.100 ±0.568）分比（2.700±0.823）分,（29.083±10.470） ng/ml比（3.908±1.984） ng/ml,（219.250±30.821） pg/ml比（140.056-27.684） pg/ml,P值均＜0.05].昆明小鼠模型组与BALB/c小鼠模型组相比较,其症状评分和血清IL-4水平差异无统计学意义[（6.100±0.568）分比（6.313 ±0.704）分,（219.250 ±30.821） pg/ml比（253.294±53.953） pg/ml,P值均＞0.05],而血清OVA-sIgE水平显著降低,差异有统计学意义[（29.083±10.470） ng/ml比（76.277±25.724）ng/ml,P＜0.05].结论 昆明小鼠通过OVA建模可以获得临床表现明显的AR模型,其血清OVA-sIgE及IL-4水平显著升高.昆明小鼠表现出很好的耐受性,该模型或可降低动物实验过程中的损耗.Objective To discusses the feasibility of establishing the allergic rhinitis （AR） model in Kunming mice.Methods Kunming mice （n =20） and BALB/c mice （n =20） were sensitized and motivated by ovalbumin （OVA） and aluminium hydroxide.The symptoms rating scale （symptoms score 〉5 as successful model） was used to evaluate AR symptoms of two kinds of mice after the AR model were set up.The differences of OVA serum specific IgE （OVA-sIgE） and the levels of interleukin 4 （IL-4） in Kunming mice model group and Kunming mice blank group were evaluated,the symptom scores,OVA serum specific IgE （OVA-sIgE） and the levels of interleukin 4 （IL-4） of successful model of Kunming mice and BALB/c mice were compared,and the difference between two kinds of animal models.SPSS 22.0 statistical software was used for statistical analysis was analysed.SPSS 22.0 statistical software was used for statistical analysis.Results During the experiment,2 mice died in BALB/c mouse model group,and no mouse died in Kunming mouse model group.Kunming model group gained a certain incease of weight.The clinical symptom scores,OVA-sIgE levels,serum IL-4 levels of Kunming mouse model group were significantly higher than those of blank control group.The difference was statistically significant [（6.100 ± 0.568） vs （2.700 ± 0.823）,（29.083 ± 10.470） ng/ml vs （3.908 ± 1.984） ng/ml,（219.250 ± 30.821） pg/ml vs （140.056 ± 27.684） pg/ml,all P 〈 0.05].Compared with BALB/c mice model group,the symptom score and serum level of IL-4 of Kunming mice model group had no statistically significant differences （6.100 ± 0.568） vs （6.313±0.704）,（219.250±30.821） pg/mlvs （253.294±53.953） pg/ml,all P〉0.05）,and the difference of serum level of OVA-sIgE was statistically significant （（29.083 ± 10.470） ng/ml vs （76.277 ±25.724） ng/rnl,P 〈 0.05）.Conclusions Kunming mice are able to obtain a clinically significant AR model through OVA modeling,with a significant increase in seru

关 键 词：鼻炎 变应性 模型 动物 小鼠 可行性研究 

分 类 号：R-332[医药卫生] R765.21