熊果酸抑制骨肉瘤细胞的生物学功能  

Biological function of inhibition of osteosarcoma cells by ursolic acid

作　　者：裴祎[1] 张晓晶[1] 邢浩[1] 郑珂[1] 商冠宁[1] 王玉名[1] 王巍[1] 邱恩铎[1] 薛峰[2] 王广斌[2] 

机构地区：[1]中国医科大学肿瘤医院辽宁省肿瘤医院骨与软组织肿瘤科,沈阳110042 [2]中国医科大学附属盛京医院骨科,沈阳110004

出　　处：《实用药物与临床》2017年第7期748-751,共4页Practical Pharmacy and Clinical Remedies

摘　　要：目的检测熊果酸对骨肉瘤细胞生长转移的影响。方法将不同浓度熊果酸分别作用于人骨肉瘤细胞系U2OS细胞,MTT实验检测细胞增殖情况,Hochest 33258染色检测细胞凋亡情况,Transwell实验检测细胞转移情况。利用Western blot方法检测熊果酸对相关蛋白的作用。结果 10、20、30μg/mL熊果酸处理U2OS细胞24 h后,对其增殖的抑制率分别为39.51%±0.75%、48.91%±3.64%、56.49%±1.54%。熊果酸通过抑制cyclin d1蛋白抑制U2OS细胞的增殖,通过调节bcl-2和bax蛋白促进U2OS细胞的凋亡。Transwell实验发现,熊果酸可以剂量依赖的方式抑制U2OS细胞的迁移,其抑制作用是通过其对MMP2蛋白的抑制实现的。结论熊果酸可以抑制骨肉瘤细胞系U2OS细胞的增殖与转移,可以作为临床骨肉瘤治疗的潜在化疗药物进行进一步探索。Objective To detect the effect of ursolic acid on the growth and metastasis of osteosarcoma cells. Methods Different concentrations of ursolic acid were applied to human osteosarcoma cell line U2OS cells. The cell proliferation was detected by MTT assay. Hochest 33258 assay was used to detect the apoptosis of U2OS cells. The cell metastasis was detected by Transwell assay. Western blot was used to detect the effect of ursolic acid on U2OS cells-as- sociated proteins. Results At 24 h after treatment with 10,20 and 30 μg/mL of ursolic acid for U2OS cells, the inhibi- tion rates of proliferation were 39.51% ±0. 75% ,48.91%±3.64% and 56.49%±1.54%. Ursolic acid could inhibit the proliferation of U2OS cells by inhibiting cyclin dl protein, and promote the apoptosis of U2OS cells by regulating bcl-2 and bax. Transwell experiments showed that ursolic acid could inhibit the migration of U2OS cells in a dose-de- pendent manner, which was achieved by the inhibition of MMP2. Conclusion Ursolic acid can inhibit the proliferation and metastasis of osteosarcoma U20S cells ,which can be further explored as potential chemotherapy drugs in osteosarcoma.

关 键 词：熊果酸 U2OS 骨肉瘤 增殖 凋亡 迁移 

分 类 号：R738.1[医药卫生—肿瘤]