小鼠间断性禁食模型的建立及其对葡萄糖内稳态的影响  

Establishment of a mouse model of intermittent fasting and characterization of its effect on glucose homeostasis

作　　者：马澜婧[1] 张浩浩[1] 丁美玲[1] 张成博[1] 倪耀宗[1] 田祖宏 唐光波[1] 窦建华[1] 聂勇战[1] MA Lan-jing ZHANG Hao-hao DING Mei-ling ZHANG Cheng-bo NI Yao-zong TIAN Zu-hong TANG Guang-bo DOU Jian-hua NIE Yong-zhan(The State Key Laboratory of Cell Biology, Xi Jing Hospital, The Fourth Military Medical University, Xi＇An 710032, China)

机构地区：[1]第四军医大学西京医院肿瘤生物学国家重点实验室,西安710032

出　　处：《中国比较医学杂志》2017年第7期24-28,共5页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金重大国际合作项目(编号:81120108005)

摘　　要：目的探寻稳定、可靠替代传统热量限制的小鼠模型建立方法及其对葡萄糖内稳态的影响。方法将40只SPF级16周龄C57BL/6J小鼠(雌雄各半)随机按性别及喂养方式分为四组。自由饮食组给予基础饲料自由取食,间断禁食组正常饮食和禁食各24 h交替进行,禁食日可自由饮水。监测各组小鼠体质量和血糖变化情况,并与建模前和建模12周行经腹腔葡萄糖耐量实验和经腹腔胰岛素耐量实验。结果小鼠间断性禁食12周后,体质量和空腹血糖未见明显变化。腹腔注射葡萄糖后血糖上升幅度减轻,腹腔注射胰岛素后血糖下降比例增加。与对照组相比,上述耐量实验曲线下面积均明显减低(P<0.05)。结论小鼠间断性禁食后胰岛素敏感性增加,糖耐量改善,且该造模方法操作简单,可作为替代传统热量限制模型的有效选择。Objective To establish a stable and reliable mouse model as an alternative to the traditional model of impaired glucose tolerance induced by calorie restriction and its effect on glucose homeostasis. Methods Forty 16-week- old SPF C57BL/6J mice （ half male and half female） were randomly divided into four groups by sex and the way of feeding. The mice in the ad libitum （AL） group had free access to basic diet, while the mice in the intermittent fasting （IF） group had normal diet and fasting on alternate days, with free access to water on the fasting days. The changes of body weight and blood glucose concentration in each group were monitored, and intraperitoneal glucose tolerance test and insulin tolerance test in mice were performed before and after the 12-week IF treatment. Results At 12 weeks after 1F treatment, the body weight and blood glucose concentration of mice did not show significant difference. After i. p. injection of glucose, the blood glucose concentration of IF mice was less increased than the AL group, and after the insulin injection, the blood glucose concentration was more decreased. Compared to the AL group, the areas under the curve of tolerance test in the IF group were significantly decreased （P 〈 0.05）. Conclusions After IF treatment, the mice show an enhanced sensitivity to insulin and improved glucose tolerance. This establishment method of mouse model of intermittent fasting is easy and simple, therefore, can be used as an effective alternative to traditional calorie restriction model of impaired glucose tolerance.

关 键 词：热量限制 间断性禁食 葡萄糖内稳态 胰岛素 

分 类 号：R-33[医药卫生]