Mac-1基因缺失对Apc^(Min/+)小鼠肿瘤生长及脂代谢的影响  

The effects of Mac-1 deficiency on tumor growth and lipid metabolism in Apc^(Min/+) mice

作　　者：章倩倩[1] 叶志金[1] 李江超[1] 王丽京[1] ZHANG Qian-qian YE Zhi-jin LI Jiang-chao WANG Li-jing(Vascular Biology Research Institute, School of Basic Course, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong, China)

机构地区：[1]广东药科大学基础学院血管生物学研究所,广东广州510006

出　　处：《广东医学》2017年第16期2425-2427,共3页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:31471290);广东省科技计划项目(编号:2015A030302083);广州市科技计划项目(编号:201610010045)

摘　　要：目的探讨整合素Mac-1基因缺失对自发肠道腺瘤小鼠Apc^(Min/+)脂代谢的影响。方法 Apc^(Min/+)小鼠和Mac-1基因缺失小鼠(Mac-1^(-/-))扩群获得实验所需数量小鼠,并进行杂交剪尾提取DNA,用PCR法检测小鼠基因型;计数肠道腺瘤数目和体积,并检测血清中总胆固醇(TC)和三酰甘油(TG)的变化。结果获得Mac-1缺失的自发腺瘤Apc^(Min/+);Mac^(-1-)/-小鼠。Mac-1基因缺失后,ApcMin/+小鼠小肠腺瘤数目和体积减少,血清中TC和TG均下调约40%。结论 Mac-1基因缺失抑制Apc^(Min/+)小鼠小肠腺瘤生长可能与脂代谢有关。Objective To investigate the influence of Mac-1 deficiency on tumor growth and lipid metabolism of spontaneous intestinal adenoma.Methods The ApcMin/＋ mice and Mac-1 knock-out （Mac-1-/-） mice were expanded.The ApcMin/＋ mice were mated with Mac-1-/-mice.The DNA was extracted and genotyped by PCR assay.The tumor number and volume were assessed, and the serum total cholesterol （TC） and triglyceride （TG） were detected.Results The Mac-1 deficiency mice with spontaneous intestinal adenoma were obtained.The tumor number and volume in small intestine were reduced.The serum TG and TC were both reduced by about 40%.Conclusion Mac-1 gene deletion could significantly suppress intestinal adenoma growth, that may correlate with lipid metabolism.

关 键 词：MAC-1 肿瘤生长 脂代谢 

分 类 号：R733.71[医药卫生—肿瘤]