线栓法大鼠脑缺血再灌注损伤模型的实验研究  

Experimental study on the suture-occluded technique for ischemia-reperfusion injury models in rats

作　　者：杨丽[1] 陈阳[1] YANG Li CHEN Yang(Department of Forensic Pathology, Wannan Medical College, Wuhu 241002, China)

机构地区：[1]皖南医学院法医病理学教研室,安徽芜湖241002

出　　处：《皖南医学院学报》2017年第4期318-321,共4页Journal of Wannan Medical College

摘　　要：目的:探讨大鼠大脑中动脉缺血再灌注(MCAO/R)损伤模型的建立及其稳定性评价,以及再灌注时间对脑梗死体积的影响。方法:SD雄性大鼠70只,随机分为假手术组(n=5)及MCAO/R组(n=65),MCAO/R组用改良的Zea-Longa法制作大鼠大脑中动脉缺血再灌注模型,观察大鼠神经功能缺损,采用Longa评分法对大鼠神经行为学进行评分。分别于再灌注3 h、6 h、12 h、24 h、2 d、3 d、5 d、7 d后断头处死大鼠,取脑组织0.2%TTC染液进行染色。测量不同时间组脑梗死体积百分比,比较各组间大鼠神经功能缺损及脑梗死体积百分比。结果:MCAO/R组大鼠造模稳定性可达86.95%;与假手术组比较,MCAO/R组大鼠均出现神经功能缺损,脑梗死体积百分比在6 h组逐渐增大,3 d组达到高峰,5~7 d组下降。结论:线栓法制作大鼠脑局部缺血再灌注损伤模型是一种稳定性好、可重复性高的造模方法;随再灌注时间的延长脑梗死体积百分比先逐渐增大,而后逐渐减小。Objective： To establish middle cerebral artery ischemia-repeffusion（MCAO/R） injury models in laboratory rats using suture-occluded technique, and assess the stability of such models as well as observe the effects of reperfusion time on cerebral infarction volume.Methods ： Seventy male Sprague-Dawley rats were randomized into sham operation group （n = 5） and MCAO/R group （n = 65）.The MCAO/R injury models were established as modi- fied Zea-Longa method,and the neurological deficits in the model rats were observed and evaluated with Longa Score Scale.Then all rats were decapitated at 3 h,6 h, 12 h,24 h, 2 d, 3 d, 5 d and 7 d, respectively following reperfusion, and the cerebral tissues were taken to undergo staining using 0.2%TTC.The percentage of cerebral infarction volume in different time phase were measured, and the percentage of cerebral infarction volume and neurological deficits were compared among different time phases.Results： The stability in MCAO/R group reached 86.95%, and rats in the MCAO/R group generally demonstrated neurologic deficits.The percentage of infarction volume was increased at 6 h,peaked at 3 d,yet declined from 5 d to 7 d .Conclusion ： Suture-occluded technique can be stable and reproductive in establishment of ischemia-reperfusion injury models in animals.The infraction volume in model rats tends to increase with prolonged reperfusion and decrease with time progression.

关 键 词：动物模型 大脑中动脉缺血再灌注 梗死体积 

分 类 号：R-332[医药卫生] R587.1