miR-375通过抑制KLF4促进前列腺癌细胞的迁移和侵袭  

miR-375 promotes prostate cancer cell migration and invasion by targeting KLF4

作　　者：潘恩山[1] 李煜罡[1] 朱晓光[1] Pan Enshan Li Yugang Zhu Xiaoguang(Department of Urology, TCM-Integrated Cancer of Southern Medical University ,Guangzhou,Guandong 510315 ,China)

机构地区：[1]南方医科大学中西医结合医院泌尿外科,广州510315

出　　处：《重庆医学》2017年第23期3184-3188,共5页Chongqing medicine

摘　　要：目的探讨miR-375在前列腺癌(PCa)细胞中的表达、作用及机制。方法培养细胞,转染质粒,Transwell分析PCa细胞的迁移和侵袭;qPCR分析miR-375、KLF4mRNA在PCa细胞中的表达;Western blot分析KLF4蛋白在PCa细胞中的表达;生物信息学分析miR-375的靶基因,双荧光素酶实验验证。结果 miR-375在PCa中高表达,抑制miR-375的表达显著抑制PCa细胞的迁移和侵袭;通过生物信息学分析miR-375的潜在靶基因含有KLF4,双荧光素酶实验验证KLF4为miR-375的靶基因;KLF4在PCa细胞中表达显著降低,抑制miR-375的表达能够显著促进KLF4蛋白的表达;进一步研究表明抑制KLF4表达可逆转miR-375抑制物对PCa细胞的迁移侵袭的抑制作用。结论 miR-375抑制KLF4的表达促进PCa的迁移和侵袭,发挥癌基因的作用;其可以作为PCa的治疗靶点,为PCa的治疗提供新的方向。Objective To explore the expression,role and mechanism of miR-375 in prostate cancer （PCa） cells. Methods PCa cells were cultured and transfected with plasmid,the migration and invasion of PCa were detected by Transwell;the expression of miR-375 and KLF4 mRNA were detected by qPCR; the expression of KLF4 were detect by Western blot; the potential target genes of miR-375 were analyzed by bioinformatics and verified by dual luciferase report. Results The expression of miR-375 were significantly up regulated in the PCa; Inhibited the expression of miR-375 could significantly inhibit the migration and invasion of PCa cells. KLF4 was the potential target genes of miR-375, which verified through bioinformaties analysis and dual luciferase report. The expression of KLF4 were significantly down regulated in the PCa. Inhibited the expression of miR-375 could significantly up-regulated the expression of KLF4. Inhibited the KLF4 expression was able to reverse the suppressive effect miR-375 has on the migration and invasion of PCa cells. Conclusion miR-375 promotes the migration and invasion of PCa via inhibiting the expression of KLF4 and play the oncogene role. MiR-375 can be used as therapeutic targets for PCa,and provide a new direction for the treatment of PCa.

关 键 词：miR-375 KLF4 前列腺肿瘤 细胞运动 肿瘤侵润 

分 类 号：R737.25[医药卫生—肿瘤]