无醛固定液与醛类固定液对细胞核DNA及细胞质着色的影响  

Effects of aldehyde-free fixation solution and aldehyde fixation solution on the nucleus DNA and cytoplasm staining

作　　者：黄荣祥 余岚岚 朱晨雁 马亮 聂琨 何文波 Huang Rongxiang Yu Lanlan Zhu Chenyan Ma Liang Nie Kun He Wenbo(X iarnen Motic Medical Laboratory , X iamen , Fuj ian 361006 ,China)

机构地区：[1]福建省厦门麦克奥迪医学检验所,361006

出　　处：《重庆医学》2017年第23期3241-3244,共4页Chongqing medicine

摘　　要：目的旨在探究无醛固定液与醛类固定液对细胞核DNA及细胞质着色的影响。方法通过运用DNA定量分析系统(ICM)对分别经过8种不同固定液固定的细胞核DNA含量(IOD)、离散系数(CV)、DNA指数(DI)、细胞核面积进行测量,联合细胞学染色,分析不同固定液对核DNA及细胞质染色的影响。结果醛类固定液优于无醛固定液,固定液中的甲醛对Feulgen-eosin联合染色有重要影响。结论采用醛类固定液进行后固定,硫堇对细胞核DNA染色,与细胞学染色相结合,获得了良好的染色效果,为后续的多技术联合诊断提供依据。Objective To investigate the effect of aldehyde-free fixation solution and aldehyde fixation solution on DNA and cytoplasm of nucleus. Methods The DNA quantification （IOD） ,discrete coefficient （CV） ,DNA index （DI） and nucleus area （area） were measured by DNA quantification system （ICM） in 8 different fixation solution; combined with cytological staining,the effects of different fixative solution on nuclear DNA and cytoplasmic staining were analyzed. Results Aldehyde fixation solution was better than aldehyde free fixation solution,the formaldehyde in fixtion solution has important influence on Feulgen-eosin staining. Conclusion Aldehyde fixation solution combined with cytological staining can obtained good dyeing effect, which provides the basis for the subsequent multi-technique joint diagnosis.

关 键 词：病理学 定量 图像细胞测定 细胞 固定化 DNA 

分 类 号：R361.2[医药卫生—病理学]