深部热疗对AD离体细胞治疗模式的探索及疗效  

The Exploration and Curative Effect of Deep Hyperthermia Treatment Model on Isolated AD Cells

作　　者：陈剑虹[1] 王小璞[1] 农海宁 鲍晋[1] 卢宜民[1] 

机构地区：[1]广州中医药大学祈福医院,广东广州511495

出　　处：《中国民族民间医药》2017年第15期58-61,共4页Chinese Journal of Ethnomedicine and Ethnopharmacy

摘　　要：目的:探索深部热疗对AD离体细胞的最佳治疗模式并评估治疗效果。方法:制备离体AD细胞模型,随机分成对照组、对照组热疗组、AD模型组、AD模型热疗组,分别置于41.5℃加热30、45、60、75、90min,于600nm处测吸光度(A)值,计算最佳热疗时间。造模成AD细胞后置于41.5℃加热60min,加热后继续置于37℃恒温孵育24h,分别于加热后2、4、8、12、24、72h取出,使用流式细胞仪行活细胞膜HSP70表达量,计算最佳热疗间隔时间。4组细胞热疗后分别加入20μmol/L的Aβ25-35溶液培养24h,应用流式细胞仪检测细胞活力,评估热疗治疗AD效果。结果:对照组和AD模型组分别给予不同时间41.5℃热疗,两组细胞从加热60min开始细胞增殖趋势与非加热细胞相比开始下降(P<0.05),这种下降趋势在75min达到最大,确定细胞加热的安全有效时间为60min,同时对照热疗组与AD模型热疗组对比,两组细胞随温度变化的细胞增殖趋势基本相同,两者比较无统计学差异(P>0.05),表明两组细胞的最佳热疗时间均为60min。AD模型组细胞在热疗后2、4、8、12、24及72h各时间段监测的HSP70表达量均不同,AD细胞在24h HSP70表达量均达到最大值(P<0.05),其后HSP70表达量开始下降,表明24h是最佳的热疗间隔时间。4组细胞与Aβ作用后加热,MTT检测表明4组细胞活力呈温度依赖性上升趋势(P<0.05),表明热疗可有效的减少Aβ的聚集,促进Aβ的清除,保护AD细胞。结论:深部热疗对AD离体细胞的最佳热疗时间为60min,最佳热疗间隔时间为24h,深部热疗可以有效的清除Aβ,促进神经元细胞修复。Objective To explore the best treatment model of deep hyperthermia on isolated AD cells and evaluate its curative effect. Methods Isolated AD cells models were made ready and randomly divided into control group and hyperthermia control group, AD model group, AD model hyperthermia group. Each group was separately heated at the level of 41.5℃ for 30, 45, 60, 75, 90min, the absor- bance （A） value was tested at 600 nm, and then the best hyperthermia treatment time was calculated. The ready made AD cells were heated for 60 minutes at the level of 41.5℃ and then continued to be incubated in thermotank for 24 hours at the level of 37℃ after the heating. The cells were separately taken out after 2, 4, 8, 12, 24 and 72h. Flow Cytometer was used to test the expression level of the AD cells HSP70, and the best interval of hyperthermia treatment was calculated. 4 groups of the cells were put into the Aβ25-3 solution of 20μmol/L concentration to cultivate for 24 hours. The cell viability was tested by using flow cytometer to evaluate the hyperthermia treat- ment effects on AD. Results The control group and AD model group were separately given hyperthermia at the level of 41.5℃, the Cell proliferation trend of the two groups of ceils began to decline compared with the non - heated cells （P 〈 O. 05）. This trend came to the maximum at the 75th minute, and accordingly the safe and effective time length of heating on the cells was set for 60 minuets. The control hyperthermia compared with the AD model hyperthermia group, the Cell proliferation trend of the two groups which varied according to the temperature changes was generally the same, and no statistic significance was found between the two groups （P 〉 0. 05 ）, which showed the best treatment time length of the two groups of ceils was 60 minutes. The HSP70 expression level of AD model group was tested differ- ently after the cells were heated 2, 4, 8, 12, 24 and 72h. The HSP70 expression level of AD cells was tested the top at 24h, then it be- gan to

关 键 词：深部热疗 AD 离体细胞 治疗模式 

分 类 号：R331[医药卫生—人体生理学]