芒果苷联合麻黄碱治疗过敏性哮喘的作用研究  

Mangiferin and ephedrine combined treatment in asthmatic mouse model

作　　者：运晨霞[1,2] 杜军 兰太进[1,3] 邓家刚 郭宏伟[4,5] 

机构地区：[1]广西中医药大学基础医学院,广西南宁530200 [2]广西医科大学研究生院,广西南宁530021 [3]广西中药药效筛选重点实验室,广西南宁530200 [4]长寿与老年相关疾病教育部重点实验室,广西南宁530021 [5]广西医科大学药学院,广西南宁530021

出　　处：《中国药理学通报》2017年第9期1314-1319,共6页Chinese Pharmacological Bulletin

基　　金：中国博士后科学基金资助项目(No 2015M582483);广西省自然科学基金资助项目(No 2015GXNSFAA139105);广西省科学研究与技术开发计划项目(No桂科攻1598013-8);广西省高校科学技术研究项目(No KY2015YB057)

摘　　要：目的探讨芒果苷联合麻黄碱治疗过敏性哮喘的作用及分子机制。方法运用OVA建立过敏性哮喘小鼠模型,观察小鼠哮喘发作时表现及给药1 h后小鼠自主活动变化;观察外周血白细胞分类并计数;HE染色观察肺组织炎症细胞浸润;ELISA法检测小鼠血清中OVA-sIgE和c AMP的含量;RT-PCR法检测小鼠肺组织中IFN-γ、IL-4、IL-5的mRNA表达。结果联合给药组和芒果苷组可明显减少由麻黄碱引起的小鼠自主活动力增强表现;各治疗组均可明显减轻小鼠哮喘发作表现,改善肺组织的病理学改变,减轻气道炎症,联合给药组效果优于芒果苷组;各治疗组均能明显减少全血中白细胞总数和嗜酸性粒细胞(EOS)比例;降低血清中OVA-sIgE含量,联合给药组作用优于芒果苷组;麻黄碱组和联合给药组可升高血清中cAMP含量;联合给药组可降低肺组织中Th2型细胞因子IL-4和IL-5 mRNA表达水平,同时上调Th1型细胞因子IFN-γmRNA表达水平。结论联合用药组平喘效果优于芒果苷组,并能减轻单用麻黄碱所引起的不良反应。其作用机制可能与调节Th1/Th2型细胞因子的失衡及升高cAMP含量相关。Aim To investigate the combined effect of mangiferin and ephedrine as well as explore its underlying mechanism in mouse model of allergic asthma.Methods The mouse model of asthma was sensitized and challenged with ovalbumin（ OVA）. The behaviors of mice during the challenging period were observed.Continuous autonomic activities of mice after 1 h of drug treatment were monitored with the autonomic activity recorder for 14 days. The total and differential cells in peripheral blood were counted. Histological study of lung sections on airway inflammation was carried out with haematoxylin and eosin（ HE） staining.The levels of ovalbumin-specific immunoglobulin E（ OVA-sIgE） and（ Cyclic Adenosine monophosphate）cAMP in serum were detected by enzyme-linked immunosorbent assay（ ELISA）. The m RNA expression of cytokines mainly produced by Th1/Th2 lymphocytes such as IFN-γ and IL-4,IL-5 were semi-quantitatively analyzed with reverse transcription polymerase chain reaction（ RT-PCR）. Results Combination treatment and mangiferin could reduce the aberrantly autonomic activity caused by ephedrine in mice. Compared with the model group,asthmatic symptoms in all treatment groups could be significantly relieved,the pathological changes of lung tissue were improved and airway inflammation was reduced. The effect of combined treatment was better than that of mangiferin treatment alone. Compared with the model group,the total number of white blood cells（ WBC） and the eosinophils（ EOS） ratio in peripheral blood in all treatment groups decreased,the level of OVA-sIgE declined and the level of cAMP increased in serum. Combination treatment could lower m RNA expressions of IL-4 and IL-5from Th2 lymphocytes and increase the m RNA expressions of IFN-γ released by Th1 lymphocytes. Conclusions The effect of combined therapy of mangiferin and ephedrine is better than that of mangiferin treated alone,meanwhile it can reduce the side effects caused by ephedrine. The underlying mechanism is mainly associated with at

关 键 词：哮喘 小鼠 芒果苷 麻黄碱 联合用药 分子机制 

分 类 号：R-332[医药卫生] R284.1