丹参花挥发油提取工艺优化及抗氧化活性研究  

Study on extraction technology of volatile oil from flower of Salvia miltiorrhiza Bge. and its antioxidant activity

作　　者：陈燕文[1] 李玉娟[1] 胡晶红[1] 席晓志 刘谦[1] 李佳[1] 郝志[1] 

机构地区：[1]山东中医药大学药学院,山东济南250355

出　　处：《山东科学》2017年第4期19-25,30,共8页Shandong Science

基　　金：山东省科技发展计划(2008GG2NS02022);山东省现代农业产业技术体系中草药产业创新团队建设项目(2015);山东省高等学校科技计划(J15LM55)

摘　　要：采用单因素法和L_9(3~4)正交试验法,优化水蒸气蒸馏法提取丹参花挥发油的提取工艺,考察不同提取溶液、浸渍时间、蒸馏时间和料液比对丹参花挥发油收率的影响;应用DPPH法研究丹参花挥发油的抗氧化能力,并比较不同提取溶液所得挥发油的自由基清除能力。结果表明,影响丹参花挥发油收率的主要因素为蒸馏时间,其次为料液比、浸渍时间;最佳工艺条件为饱和氯化钠溶液、浸渍6 h、蒸馏8 h、料液比为1∶18,此条件下丹参花挥发油的收率为0.0935%。DPPH实验表明丹参花挥发油具有显著的抗氧化能力;在一定浓度范围内其抗氧化能力与挥发油浓度呈正相关性,蒸馏水、饱和氯化钠溶液、pH=1的硫酸水和pH=1的饱和氯化钠硫酸水4种提取液所得挥发油对DPPH的IC_(50)值分别为1.07、1.03、1.45、1.40 mg/m L。通过验证实验表明,优化所得丹参花挥发油提取工艺稳定、易行。Through single factor experiment and L9（3^4） orthogonal test,the extraction process of volatile oil from flower of S. miltiorrhiza. by steam distillation was optimized and the effects of different extraction solutions,soaking time,extracting time,and solid-to-liquid ratio on the yield of the volatile oil from the flower of S. miltiorrhiza. were investigated. The antioxidant capacity of volatile oil from S. miltiorrhiza flower was evaluated by DPPH method,and the free radical scavenging capacities of volatile oils from different extraction solutions were compared. The results showed that the primary factors affecting the yield of volatile oil from S. miltiorrhiza flower were distillation time,followed by solid-liquid ratio and soaking time. The optimum extraction conditions were as follows： saturated sodium chloride solution,soaking 6 hours,extracting 8 hours,solid-to-solvent ratio 118（ g/m L）. Under this condition,the extraction rate of volatile oil from flower of S. miltiorrhiza was 0. 0935%. The DPPH assays showed that volatile oil from flower of S. miltiorrhiza has significant antioxidant capacity. In a certain concentration range,antioxidant capacity was positively correlated with the concentration of volatile oil from flower of S. miltiorrhiza. In the DPPH assays,the IC50 values of volatile oil extracted by four kinds ofextraction solution（ distilled water,saturated sodium chloride solution,aqueous sulfuric acid of pH = 1,saturated sodium chloride and aqueous sulfuric acid of pH = 1） were 1. 07,1. 03,1. 45 and 1. 40 mg/m L,respectively. The results showed that the optimized extraction process of volatile oil from flower of S. miltiorrhiza was stable and practicable.

关 键 词：丹参花 挥发油 提取工艺 DPPH 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]