基于高通量测序的药用植物“凤丹”根皮的转录组分析  

Next generation sequencing and transcriptome analysis of root bark from Paeonia suffruticosa cv. Feng Dan

作　　者：谢冬梅[1,2] 俞年军[1] 黄璐琦[2] 彭代银[1] 刘丛彬[1] 朱月健[3] 黄浩 

机构地区：[1]安徽中医药大学安徽省中医药科学院中药资源保护与开发研究所,安徽合肥230012 [2]中国中医科学院中药资源中心道地药材国家重点实验室培育基地,北京100700 [3]安徽济人药业有限公司,安徽亳州236800 [4]北京同仁堂安徽中药材有限公司,安徽铜陵244000

出　　处：《中国中药杂志》2017年第15期2954-2961,共8页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81503190);安徽高校科研创新平台团队项目(皖教秘科[2015]49号);安徽道地中药材品质提升协同创新中心项目(皖教秘科[2014]44号)

摘　　要：牡丹皮为我国传统常用中药,安徽省铜陵地区栽培的"凤丹"根皮加工而成的药材牡丹皮被誉为道地药材,药用活性成分丰富多样,但目前尚不清楚"凤丹"药用部位次生代谢过程中活性物质合成的遗传学基础。研究采用Illumina Hi Seq 4000高通量测序平台对五年生"凤丹"根皮转录组进行测序,对测序结果进行de novo拼接和功能注释,测序后获得72 997条unigene。进一步利用公共数据库进行同源比对,其中41 139条unigene被Nr数据库成功注释,34 952条unigene能被GO数据库成功注释,20 016条unigene被KEGG数据库成功舒注释,共涉及到5个大类、34个种类、352条代谢通路;在次生物质合成与代谢途径中,其中苯丙素类化合物、萜类化合物骨架合成、各种类型萜类化合物、生物碱类化合物以及黄酮类成分生物合成途径中的unigene分别有214,104,152,55,36个;不同产地样本间差异表达基因的富集性比较显示不同产地样本间存在明显差异;此外,在72 997条unigene中共检测到9 939个SSR序列,其中二核苷酸重复的SSR标记占20.75%。研究的结果不仅为挖掘"凤丹"次生代谢物生物合成关键基因提供了基础数据信息,也为药用牡丹的遗传多样性研究和分子标记开发奠定了分子基础。Moutan Cortex is an important traditional Chinese medicine, ＂Fengdan Pi＂ was known as Dao-di herbs from the root bark of Paeonia suffruticosa cv. Feng Dan for its extracted various active components. However, the genetic basis for their activity is virtually unknown. The transcriptome of the root bark from ＂Fengdan＂ was sequenced using the Illumina HiSeq 4000 sequencing plat- form. The clean reads were then de novo assembled into 72 997 unigenes. Among them, the number of unigenes which could been an- notated by dataset Nr and GO was 41 139 and 34 592. The 20 016 unigenes could been annotated by KEGG dataset, which were in- volved in 5 major categories, 34 middle categories, and 352 metabolism pathways. The number of unigenes which were mapped to the phenylpropanoid biosynthesis pathway, terpenoid backbone biosynthesis pathway, terpenoid biosynthesis pathway, alkaloid biosynthesis pathway, and flavonoid biosynthesis pathway was 214, 104, 152, 55 and 36 respectively, suggesting that they are involves in these pathways of pharmaceutically important. Furthermore, there also showed remarkable differences in groups which enrichment ratio of the different expressed gene compared. In addition, a total of 9 939 SSRs were identified from the sequence of 72 997 unigenes. This study not only provides many valuable basal data which was important gene in the synthesis pathway of secondary metabolites with gene searching, but also has important significance to find molecular marker in germplasm for breeding and improvement.

关 键 词：牡丹皮 转录组 次生代谢 差异基因 简单重复序列 

分 类 号：S567.15[农业科学—中草药栽培]