雪峰虫草的生物学特性及其人工培育  

Biological characteristics and cultivation of Ophiocordyceps xuefengensis

作　　者：邹娟[1,2] 伍陵 何正蜜 张平[1] 陈作红[1] 

机构地区：[1]湖南师范大学生命科学学院,湖南长沙410081 [2]怀化学院生物与食品工程学院民族药用植物资源研究与利用湖南省重点实验室,湖南怀化418000

出　　处：《菌物学报》2017年第8期1104-1110,共7页Mycosystema

基　　金：湖南省科技厅重大专项(2014FJ1007)~~

摘　　要：雪峰虫草Ophiocordyceps xuefengensis为近年来发现的真菌新物种,它是生长在活体植物大青的根或者茎中的疖蝙蛾幼虫体上的寄生菌。本文报道了从天然雪峰虫草子座中分离获得菌株,从培养性状、形态特征、分子证据(ITS序列)等方面对该菌株进行了鉴定并确证为雪峰虫草的无性型。PCR检测表明该菌株含有MAT1-2交配型基因,但不含MAT1-1交配型基因。我们成功培育了雪峰虫草子座。The anamorphic state of Ophiocordyceps xuefengensis, a recently described species of Ophiocordyceps associated withlarvae of Phassus nodus （Hepialidae） in living root or trunk of Clerodendrum cyrtophyllum, was isolated from the stroma of thefungus. Cultural and morphological characteristics, as well as molecular evidence （ITS sequence） have proved its identity. Themating-type gene MAT1-2 was amplified by polymerase chain reaction （PCR）, however, MAT1-1 gene was not determined. Thefungus bearing stroma was cultivated successfully in artificial media.

关 键 词：形态特征 ITS 子座 交配型 

分 类 号：S567.3[农业科学—中草药栽培]