^125Ⅰ粒子串近程放射治疗兔植入性门静脉主干癌栓实验研究  

Brachytherapy with ^(125)Ⅰ seed strand for the treatment of implanted main portal vein tumor thrombus:an experimental study in rabbits

作　　者：陶赟[1] 李文会[2] 刘清欣[1] 罗剑钧[1] 张雯[1] 马婧嶔 杨敏捷[1] 瞿旭东[1] 颜志平[1] 刘凌晓[1] 王建华[1] 

机构地区：[1]复旦大学附属中山医院介入科,上海市影像医学研究所,上海200032 [2]江苏省盐城市第三人民医院介入科

出　　处：《介入放射学杂志》2017年第8期727-731,共5页Journal of Interventional Radiology

基　　金：上海市卫生计生委科研项目(201540272);上海市青年科技英才扬帆计划项目(17YF1417500)

摘　　要：目的评估^(125)Ⅰ粒子串近程放射治疗兔植入性门静脉主干癌栓(MPVTT)的安全性和有效性。方法对32只新西兰大白兔门静脉主干植入VX_2肿瘤细胞株,随机分为治疗组(T组,n=16)和对照组(C组,n=16)。治疗组、对照组兔MPVTT中分别植入^(125)Ⅰ粒子串、无放射性粒子串,术后定期监测兔一般情况、体重、实验室检查变化,术后2周治疗组、对照组各处死8只兔,作病理学检查。余下兔饲养至死亡作尸检,对比两组多层螺旋CT检查结果、组织病理学检查结果、Ki-67标记指数、凋亡指数,并以此为基础评估该疗法的有效性。结果近程放射治疗后每个观察时间点均表明,对照组兔体重下降更加明显,两组兔肝功能、血细胞计数差异均无统计学意义(P>0.05)。治疗组、对照组平均MPVTT体积为分别为(565.4±220.9)mm^3、(2 269.9±437.0)mm^3,差异有显著统计学意义(P<0.001);Ki-67蛋白指数分别为(4.14±1.84)%、(33.82±6.07)%,凋亡指数分别为(6.51±1.92)%、(0.91±0.26)%,差异均有显著统计学意义(P=0.001);平均生存时间分别为(39.50±2.37)d、(27.38±1.22)d,差异有显著统计学意义(P=0.001)。结论^(125)Ⅰ粒子串近程放射治疗兔植入性MPVTT安全有效。Objective To evaluate the safety and efficacy of brachytherapy with ^125I seed strand in treating implanted main portal vein tumor thrombus （MPVTF） in experimental rabbits. Methods VX2 tumor cell line was implanted in the main portal vein （MPV） of 32 New Zealand white rabbits to establish MPVTr models. The rabbits were randomly divided into the treatment group （group T, n=16） and the control group （group C, n=16）. ^125I seed strand was implanted in the MPVTT of the rabbits of group T, while blank seed strand was implanted in the MPVTY of the rabbits of group C. After the implantation, the changes in general condition, body weight and laboratory testing results were recorded. Two weeks after the treatment, every 8 rabbits from each group were sacrificed, and the specimens were collected and sent for pathological examination. The remaining rabbits were fed till they died, and then autopsy was conducted. Muhi-slice spiral CT manifestations, histopathological findings, Ki-67 labeling index and apoptosis index were used to assess the curative effect, and the results were compared between the two groups. Results At each observation time point after brachytherapy, the weight loss of the experimental rabbits was more obvious in group C than in group T. No statistically significant differences in liver functions and white blood cell count existed between the two groups （P〉0.05）. The mean MPVTF volume of group T and group C were （565.40±220.90） mm3 and （2 269.90±437.00） mm3 respectively （P〈0.001）; the Ki-67 labeling indexes were （4.14±1.84）% and （33.82 ±6.07）% respectively （P=0.001） ; the median survival days were （39.50±2.37） d and （27.38±1.22） d respectively （P=0.001）. Conclusion For the treatment of implanted MPVTY in experimental rabbits, brachytherapy with ^125I seed strand is safe and effective. （J Intervent Radiol, 2017, 26： 727-731）

关 键 词：近程放射治疗 ^125I粒子 VX2肿瘤细胞株 门静脉主干癌栓 

分 类 号：R735.7[医药卫生—肿瘤]