小鼠脑爆震伤中PI3K/Akt通路及相关凋亡蛋白表达情况  

Expression of PI3K/Akt pathway and related apoptotic proteinsinmouse braininduced by traumatic shock

作　　者：史秀云[1] 柳云恩[1] 张玉彪[1] 佟昌慈 施琳[1] 丛培芳[1] 刘颖[1] 金红旭[1] 侯明晓[1] 

机构地区：[1]沈阳军区总医院急诊医学部全军重症(战)创伤救治中心实验室辽宁省重症创伤和器官保护重点实验室,辽宁沈阳110016

出　　处：《创伤与急危重病医学》2017年第4期215-219,共5页Trauma and Critical Care Medicine

基　　金：全军十二五面上项目(CSY12J002);全军十二五面上项目(CSY13J002);全军重大新药创制项目(2013ZX09J13109-02B);总后卫生部重大新上(ASM14L008)

摘　　要：目的探讨小鼠脑爆震伤后不同时间点磷脂酰肌醇3-激酶/蛋白激酶B(PI3K/Akt)通路及相关凋亡蛋白Bcl-2、Bax的表达情况。方法将50只雄性小鼠通过随机数字表法分为对照组(n=10)和模型组(n=40),再将模型组根据造模成功后时间分为3 h组、6 h组、12 h组和24 h组,每组各10只。测定各组脑组织含水量与伊文思蓝含量,并采用Western blot以及实时荧光定量PCR等技术检测各组小鼠脑组织中PI3K、Akt、Bcl-2、Bax的含量和m RNA的表达变化。结果模型组各时间点脑组织含水量与伊文思蓝含量较对照组均明显升高,差异有统计学意义(P<0.05)。Western blot检测与实时荧光定量PCR检测结果一致。Akt、PI3K和Bcl-2表达,3 h组、6 h组均较对照组显著降低,6 h组达到最低,差异均有统计学意义(P<0.05);12 h组、24 h组较6 h组有所升高,但仍低于对照组,且与对照组比较,差异无统计学意义(P>0.05)。Bax表达,3 h组、6 h组、12 h组均较对照组显著升高,差异均有统计学意义(P<0.05);24 h组较12 h组有所降低,但仍高于对照组,且与对照组比较,差异无统计学意义(P>0.05)。结论颅脑爆震伤中PI3K/Akt通路和凋亡蛋白Bcl-2、Bax的表达变化对其判断和治疗有一定提示作用。Objective To investigate the expression of PI3K/Akt pathway and related apoptosis proteins Bcl-2 and Bax at different time points after blast injury. Methods A total of 50 experimental male rats were randomly divided into the con- trol group（ n = 10）and the model group（ n = 40）. The rats in the model group were divided into the group of 3 hours ,6 hours and 24 hours ,with 10 rats in each group. Brain water content and Evans blue content were detected in brain tissues. West- ern-blot and Real-time PCR methods were used for determination of PI3 K, Akt, Bcl-2 and Bax content and expression chan- ges of mRNA in mouse brain tissues. Results The brain water content and Evans blue content in model groups at each time point were significantly improved after modeling, and the differences between the groups had statistical significance （ P 〈 0. 05 ）. Compared with the control group, the expression of PI3 K, Akt and anti-apoptotic factor Bcl-2 were significantly lower than those of the control group, and the expression in groups of 12 hours and 24 hours increased back to normal（ P 〈 0. 05） ; the expression of pro-apoptotic factor Bax was significantly increased （ P 〈 0. 05 ）. Conclusion The changes of PI3K/Akt pathway and Bcl-2 and Baxmayhelpdetermine and carry out treatment of craniocerebralblast injury,

关 键 词：颅脑爆震伤 磷脂酰肌醇3-激酶/蛋白激酶B通路 BCL-2 BAX 

分 类 号：R737.31[医药卫生—肿瘤]