人工寒潮对易卒中型肾血管性高血压大鼠内皮素及降钙素基因相关肽的影响  

Effect of artificial cold wave on endothelin and calcitonin gene-related peptide in stroke-prone rats with renovascular hypertension

作　　者：夏勇[1] 袁忠民[1] 陈永洪[1] 傅君毅 吴钶坪 柯冠豪 解龙昌[1] XIA Yong YUAN Zhong-min CHEN Yong-hong et al(Department of Neurology, Second Affiliated Hospital of Guangzhou Medical University, C-uangzhou 510260, Guangdong Province, China)

机构地区：[1]广州医科大学附属第二医院神经内科,510260

出　　处：《中华老年心脑血管病杂志》2017年第8期867-871,共5页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：广东省科技计划(2014A020212604)

摘　　要：目的探讨人工寒潮对易卒中型肾血管性高血压大鼠内皮素及降钙素基因相关肽(CGRP)影响的研究。方法选择健康雄性SD大鼠72只,其中模型组36只,对照组36只。将2组大鼠置于人工寒潮环境中,分别将寒潮前、寒潮3、6、12h及寒潮后12、24h大鼠麻醉,每时间点6只。取嘴侧至尾侧1.5mm脑片进行内皮素、CGRP检测,原位杂交检测内皮素mRNA表达。结果模型组寒潮前,寒潮3、6、12h及寒潮后12、24h内皮素和内皮素mRNA表达明显高于对照组,寒潮前及寒潮后24hCGRP表达明显低于对照组,差异有统计学意义(P<0.05)。模型组寒潮3、6h内皮素和CGRP表达较寒潮前明显升高,差异有统计学意义(P<0.05)。模型组寒潮3、6、12h内皮素mRNA表达较寒潮前明显升高(28.00±6.02、27.06±5.46、26.40±4.60 vs 22.81±4.35,P<0.05)。结论寒潮时易卒中型肾血管性高血压大鼠内皮素及CGRP增加,与寒冷时脑卒中的发生有一定的关系。Objective To study the effect of artificial cold wave on endothelin（ET）and calcitonin gene-related peptide（CGRP）in stroke-prone rats with renovascular hypertension.Methods Seventy-two male SD rats were divided into stroke-prone renovascular hypertension group as a model group（n=36） and control group（n=36）.The animals in two groups were exposed to artificial cold wave.Brain tissue samples were taken brfore exposure to artificial cold wave,at 3,6 and 12 h during exposure to artificial cold wave,at 12 and 24h after exposure to artificial cold wave respectively and were then cut into 1.5mm-thick sections.The expressions of ET,CGRP and ET mRNA were detected by in situ hybridization.Results The expression level of ET and ET mRNA was significantly higher in model group than in control group before exposure to cold wave,at 3,6,12 h during exposure to cold wave,at 12 and 24h after exposure to cold wave while that of CGRP was significantly lower in model group than in control group before exposure to cold wave and at 24 h after exposure to cold wave（P〈0.05）.The expression level of ET and CGRP was significantly higher in model group at 3 and 6h after exposure to cold wave（P〈0.05）.The expression level of ET mRNA was significantly higher in model group at 3,6 and 12 h after exposure to cold wave than before exposure to cold wave（28.00±6.02,27.06±5.46,26.40±4.60 vs 22.81±4.35,P〈0.05）.Conclusion Elevated expression of ET and CGRP in stroke-prone rats with renovascular hypertension is related with stroke when they are exposed to cold wave.

关 键 词：高血压 肾血管性 受体 降钙素基因相关肽 寒冷 卒中 

分 类 号：R743.3[医药卫生—神经病学与精神病学]