乳腺癌及乳腺良性病变的流式细胞术检测对比分析  

Contrast analysis of breast cancer and benign lesion with FCM

作　　者：于志勇[1] 国前[1] 王永胜[1] 左文述[1] 蔡淑萍[1] 张品亮[1] 卓培英[1] 张德贤[1] 

机构地区：[1]山东省肿瘤防治研究院乳腺病中心,山东济南250117

出　　处：《中国癌症杂志》2002年第4期296-300,共5页China Oncology

摘　　要：目的 :探究乳腺癌与癌旁组织、乳腺增生症 (乳增症 )及正常乳腺组织的异倍体率、细胞增殖活性(SPF)和 p5 3、bcl 2表达与乳腺癌的发生、发展的关系。 方法 :用流式细胞术 (FCM )对 38例乳腺癌、33例癌旁组织、2 0例乳增症和 19例正常乳腺组织 ,进行DNA倍体和SPF及bcl 2和 p5 3的表达阳性细胞率检测。 结果 :癌组织与癌旁组织、乳增症及正常组织三组间异倍体阳性率及DNA指数 (DI)各有显著性差异 (P <0 .0 1) ;癌组织与癌旁组织、乳增症及正常组织间SPF各有显著性差异 (P <0 .0 1) ,癌旁组织与正常组织间有显著性差异 (P <0 .0 1) ;癌组织与癌旁组织、乳增症各组间bcl 2表达无显著性差异 (P >0 .0 5 ) ,仅癌组织与正常组织间有显著性差异 (P <0 .0 1) ;癌组织与癌旁组织、乳增症及正常三组间P5 3表达各有显著性差异 (P <0 .0 1) ;DI分别与SPF和P5 3显著相关 (P <0 .0 1) ,SPF和P5 3显著相关 (P <0 .0 1)。结论 :P5 3和bcl 2与乳腺癌的发生有一定关系 ;异倍体率与P5 3可以起到肿瘤标志物的作用 ;FCM可以作为病理诊断的补充 ;SPF与异倍体率联合分析乳腺良、恶性病变的分子生物学行为更有意义 ;尚不足以证明乳增症为癌前期病变。Purpose:To study the mechanism of breast corcinogenesis using breast cancer tissue(BC) , para cancer tissue(PC)), breast hyperplasia(BH)and normal breast tissue(NB) through the measurement of DNA content, S phase fraction (SPF) , bcl 2 and p53 protein expression.Methods:DNA content, SPF , bcl 2 and p53 protein expression were measured on 38 cases BC , 33 cases PC, 20 cases BH and 19 casses NB using flow cytometry (FCM). Results:Our results showed that the ratio of aneuploidy of BC and DI were obviously higher than those of PC,BH and NB respectively ( P <0.01). In SPF, there was significant difference statistically between BC and PC, BC and BH, BC and NB respectively ( P <0.01). There was significant difference statistically between PC and NB( P <0.01). In positive bcl 2 expression,there was significant difference statistically between BC and NB( P <0.01). In positive p53 expression, BC was markedly higher than that of PC, BH and NB respectively( P <0.05. DI was associated with SPF and p53 statistically( P <0.05), and there was significant relationship between the last two( P <0.01).Conclusions:p53 and bcl 2 might be associated with the carcinogenesis of the breast,ratio of aneuploidy and p53 might be regarded as tumor markers. FCM could be a supplementary method for pathological diagnosis. The analysis of molecular biology in breast lesion is more significant through the union of SPF and ratio of aneuploidy. There was no evidence at the moleucular level that BH was a pre malignant lesion.

关 键 词：乳腺癌 乳腺增生症 BCL-2基因 P53基因 流式细胞术 肿瘤标志物 

分 类 号：R737.9[医药卫生—肿瘤] R655.8[医药卫生—临床医学]