肺癌患者血中循环癌细胞端粒酶活性的表达及意义  

Expression of telomerase activity in peripheral blood of patients with lung cancer

作　　者：冯光丽[1] 冯久贤[2] 董强刚[2] 陈玉蓉[1] 

机构地区：[1]上海市胸科医院肺内科,上海200030 [2]上海市胸科医院肿瘤研究室,上海200030

出　　处：《中国癌症杂志》2002年第4期336-337,共2页China Oncology

摘　　要：目的 :检测肺癌患者外周血循环癌细胞端粒酶活性变化与临床资料的关系。方法 :外周血经Ficoll梯度离心分离单个核细胞组份 ,再应用PCR TRAP法检测 4 9例肺癌化疗前患者外周血中端粒酶活性。结果 :4 9例肺癌患者中 32例端粒酶活性明显升高。阳性率达 6 5 3% ,其中小细胞肺癌端粒酶活性表达阳性率最高而腺癌、鳞腺癌较低 ,同时晚期初治患者端粒酶活性明显高于复治患者。结论 :端粒酶活性变化与病理类型 ,晚期复治病人有关 ,值得进一步探索。Purpose:To detect the telomerase activity of lung cancer cells in peripheral blood by TRAP.Methods:The mononuclear cell fraction in peripheral blood was isolated by Ficoll Hypaque gradient centrifugation. Then the telomerase activity of cancer cells in peripheral blood of 49 pre chemotherapy patients was determined by PCR TRAP. Results:In the 49 cases, 65.3%(32/49) cases showed significantly increased telomerase activity, among these cases the telomerase activity was especially high in small cell lung cancer and advanced non small cell lung cancer patients, and telomerase activity in the primary chemotherapy patients was higher than in the recurrent chemotherapy patients. Conclusions:Telomerase activity may be a tumor marker of cancer cells in peripheral blood and can evaluate chemotherapy response in lung cancer.

关 键 词：肺癌 肺肿瘤 端粒酶 肿瘤标志物 循环癌细胞 

分 类 号：R734.2[医药卫生—肿瘤]