miR-92a调节MMP-2/9的表达对胃癌细胞增殖和迁移的影响及机制研究  

Effect and mechanism of miR-92a on the proliferation and migration of gastric cancer cells by regulating the expression of MMP-2/9

作　　者：戴维[1] 张瑞鹏[1] 郭维[1] 李楠[2] 

机构地区：[1]陕西省人民医院,陕西西安710068 [2]西安市儿童医院,陕西西安710043

出　　处：《临床医学研究与实践》2017年第24期1-4,共4页Clinical Research and Practice

基　　金：陕西省自然科学基础研究计划项目(No.2014JM4122)

摘　　要：目的探讨miR-92a调节MMP-2/9的表达对胃癌细胞(BGC-823)增殖和迁移的影响,并研究其可能的分子机制。方法收集IIIB期胃癌患者血液及胃癌组织样本,检测miR-17~92基因簇在胃癌IIIB期患者血液及病变胃癌组织中的表达情况,检测重组腺病毒介导SIRT1及MMP-2/9在BGC-823细胞的表达。结果ⅢB期胃癌患者血液及胃癌组织样本中的miR-92a表达显著增高;BGC-823细胞中miR-92a表达高于miR-17~92基因簇的其他成员;过表达反义miR-92a抑制BGC-823细胞的增殖和迁移能力;反义miR-92a的过表达增加SIRT1的蛋白表达,抑制MMP-2/9的蛋白表达;miR-92a通过SIRT1调节MMP-2/9的表达从而影响胃癌细胞的增殖和迁移。结论 miR-92a可通过SIRT1调节MMP-2/9的表达来影响BGC-823细胞的增殖和迁移。Objective To investigate the effect of miR-92a on the proliferation and migration of gastric cancer cells （BGC-823） by regulating the expression of MMP-2/9, and to investigate the molecular mechanism. Methods The blood and gastric cancer lesion samples in patients with stage IIIB gastric cancer were collected and the expression of miR-17-92 in serum and gastric cancer tissue were detected. The expression of recombinant adenovirus mediated SIRT1 and MMP-2/9 in BGC-823 cells were detected. Results The expression of miR-92a in the samples of blood and gastric cancer tissues in patients with stage IIIB gastric cancer was significantly increased. The expression of miR-92a in BGC-823 cells was higher than other members of miR-17-92 gene cluster. Overexpression of anti-miR-92a inhibits proliferation and migration of BGC-823 cells. Overexpression of anti-miR-92a increased the expression of SIRT1 protein and inhibited the expression of MMP -2/9 protein. MiR -92a regulated the expression of MMP -2/9 through SIRT1, and affected the proliferation and migration of gastric cancer cells. Conclusion MiR-92a can regulate the expression of MMP-2/9 through SIRT1, and affect the proliferation and migration of BGC-823 cells.

关 键 词：胃癌 miR-92a SIRT1 MMP-2/9 

分 类 号：R735.2[医药卫生—肿瘤]