一株耐盐耐氧反硝化菌MCW148的分离鉴定及其脱氮特性  

Isolation and Identification of a Salt-tolerant and Aerobic Denitrification Bacterial Strain MCW148 and Its Denitrification Characterization

作　　者：姜天翔[1] 任华峰[1] 陈进斌[1] 苗英霞[1] 邱金泉[1] 王静[1] 

机构地区：[1]国家海洋局天津海水淡化与综合利用研究所,天津300192

出　　处：《湖北农业科学》2017年第13期2442-2445,共4页Hubei Agricultural Sciences

基　　金：中央级公益性科研院所基本科研业务费专项资金项目(K-JBYWF-2016-G11;K-JBYWF-2015-G18);海洋公益性行业科研专项经费项目(201505028-5)

摘　　要：从渤海某海水养殖场(北美白对虾)底泥中分离到1株耐盐高效好氧反硝化细菌MCW148,经过对其形态特征、生理生化以及16S r DNA序列分析,将该菌株初步鉴定为巨大芽孢杆菌(Bacillus megaterium)。进一步研究表明,菌株MCW148的最适碳源为葡萄糖,最适培养温度为35℃,最适pH为6。在最适条件下,菌株MCW148在12 h对NO3--N的去除率为62.4%。A aerobic denitrification bacterial strain MCW148 with sal t-tolerance and high denitrification ability was isolated rom sediment of shrimp pond in the Bohai Sea. Results indicated this strain was identified as Bacillus rnegoterium according o its morphology, biochemical properties and 16S rDNA sequence analysis. Further study indicated the optimal carbon was glucose , and the optimal pH and temperature for cell growth and denitrification were 6.0 and 35 益 ,respectively. And the N〇3--N removal rate of the strain MCW148 was 62.4% in the optimal conditions for 12 h.

关 键 词：好氧反硝化菌 巨大芽孢杆菌(Bacillus megaterium) 脱氮 耐盐 

分 类 号：X172[环境科学与工程—环境科学]