糖尿病对直结肠癌小鼠生存的影响  

The Effects of Diabetes on Straight Colon Cancer Survival and Prognosis of Mice

作　　者：刘虹[1] 龚志军[1] 卿笃桔[1] 李恩就[1] 黎飞[1] 

机构地区：[1]江门市中心医院胃肠外科,广东江门529000

出　　处：《系统医学》2016年第2期56-59,共4页Systems Medicine

摘　　要：目的探讨糖尿病对直结肠癌小鼠生存及预后的影响。方法 BALB/c小鼠60只,观察时间2013年3月—2013年4月,共4周,小鼠饲养于中山大学北校区实验动物中心,随机分为3组,每组20只,分别为A组:结直肠癌组,B组:糖尿病结直肠癌组,C组:对照组。B组小鼠腹腔注射四氧嘧啶建立高血糖模型。体外培养小鼠结肠癌细胞株CT26至对数生长期,制成单细胞悬液。并将此细胞悬液注入A、B组小鼠皮下,C组小鼠在皮下注入生理盐水。观察各组小鼠的生存时间、体重差、并发症(肠梗阻、肠粘连、血性腹水等)的发生率及时间。建模成功4周后处死存活的小鼠,取静脉血测定肌酐、尿素氮、谷丙转氨酶和谷草转氨酶以明确肝肾功能,解剖、大体观察皮下肿瘤的生长和浸润,病理学检查是否有肝脏转移及转移瘤数量。结果处死前称重,A、B组小鼠体质量分别为(15.38±3.39)g和(14.65±2.59)g,与造模前相比明显减轻(A:19.23±0.25 g,B:21.15±0.33 g),差异有统计学意义(P<0.05),但A、B组之间的差异无统计学意义(P>0.05)。3组小鼠的肝肾功能与造模前相比无明显变化。A、B组注射部位皮下肿瘤体积比造模前明显增大,差异有统计学意义(P<0.05),且B组体积大于A组。结论糖尿病能促进结直肠癌肿瘤的生长和转移,增加并发症的发生率和严重程度,并可能提高死亡率。Objective To explore the effects of diabetes on straight colon cancer survival and prognosis of mice. Methods Sixty mice were randomly divided into 3 groups(each group was 20). A: Colorectal Cancer Group; B: Diabetes, colorectal cancer group; C: control group. Mice in B group were abdominal injection of alloxan to set up hyperglycemia model. Colon cancer cell lines mice CT26 was cultured in vitro to logarithmic phase, prepared the single cell suspension, and subcutaneous injection to the mice of A and B group. Mice in C group were subcutaneous injection of saline. The survival time,weight and complications(intestinal obstruction, intestinal adhesion, bloody ascites, etc) were observed. Four weeks after the models were successful, mice were executed, and urea nitrogen, creatinine, alanine aminotransferase and aspertate aminotransferase of venous blood were tested to evaluate the function of liver and kidney. Anatomy and gross observation the growth and infiltration of the subcutaneous tumor, pathology check to explicit whether there were liver metastasis and number of metastases. Results All mice were weighed before death, the body mass was [(15.38±3.39)g] and [(14.65±2.59)g].Compared with before the model [(19.23±0.25)g VS(21.15±0.33)g ], the differences were statistically significant(P<0.05),and there was no statistical significance between the 2 groups(P >0.05). The liver and kidney function of three groups of mice has no obvious change compared with before modeling. The subcutaneous tumor size at the injection site of A and B group was significantly larger than they did before modeling, and the difference was statistically significant(P < 0.05). And the volume of group B was greater than the group A. Conclusion Diabetes can promote tumor growth and metastasis of colorectal cancer, increases the incidence of complications and there severity, and may increase mortality.

关 键 词：糖尿病 结直肠癌 小鼠 并发症 预后 

分 类 号：R735[医药卫生—肿瘤]