电针对APP/PS1双转基因小鼠学习记忆能力及LC3 Ⅱ、P62表达水平的影响  

Effect of electroacupunctureure intervention on learning and memory ability,LC3 Ⅱ and P62 Expression level of APP/PS1 transgenic mice

作　　者：高杨[1] 李丽娜[1] 毛颖秋[1] 周英奕 张学婷[1] 张磊[1] 薛卫国[1] GAO Yang LI Lina MAO Yingqiu ZHOU Yingyi ZHANG Xueting ZHANG Lei XUE Weiguo(Beijing University of Chinese Medicine, Beijing 100029, China)

机构地区：[1]北京中医药大学,北京100029

出　　处：《长春中医药大学学报》2017年第4期523-526,共4页Journal of Changchun University of Chinese Medicine

基　　金：国家自然科学基金资助项目(81273826)

摘　　要：目的研究电针对APP/PS1双转基因小鼠自噬相关蛋白LC3 Ⅱ及受体蛋白P62相关的作用机制。方法将7月龄APP/PS1双转基因小鼠随机分为模型组、电针治疗组,以同窝转基因阴性小鼠为正常对照组。电针治疗组予电针涌泉、百会,15 min/次,隔日1次,治疗6周,利用Morris水迷宫检测各组小鼠学习记忆能力以及用Western blot法检测小鼠海马区域LC3 Ⅱ及P62蛋白的表达。结果水迷宫结果显示,模型组与正常对照组比较,逃避潜伏时增加(P<0.01);WB结果显示,与对照组相比,模型组LC3 Ⅱ表达增强,P62表达减弱(P<0.05);与模型组相比,针刺组LC3 Ⅱ表达减弱,P62表达增强(P<0.05)。结论 APP/PS1转基因鼠存在神经元自噬途径功能亢进,电针可能通过抑制自噬水平,从而改善学习记忆功能。Objective To investigate the effect of electroacupunctureure on learning and memory ability and its mechanism related to autophagic receptor P62 in APP/PS1 Transgenic mice. Methods The 7-monthold APP/PS1 transgenic mice were randomly divided into model group and EA treatment group and negative transgenic mice were selected into the nomal control group. EA was applied to＂Baihui ＂and＂Yongquan ＂for 15 min,once every other day for 6 weeks.The learning-memory ability was detected by using Morris water maze testing, hippocampal LC3 Ⅱ and P62 were detected using Western blot（WB）. Results of Morris water maze test showed that the escape latency of model group increased compared with the control group（P〈0.01）, WB results showed that compared with the control group, the model group LC3 Ⅱ expression enhanced, P62 expression decreased（P〈0.05）; compared with the model group, EA group LC3 Ⅱ expression was decreased, P62 expression increased（P〈0.05）. Conclusion EA intervention can improve learning and memory abilities in APP transgenic model mice, which may contribute to its effect in inhibiting neuronal autophagy.

关 键 词：阿尔茨海默病 APP/PS1 LC3 Ⅱ P62 

分 类 号：R245.31[医药卫生—针灸推拿学]