活体成像动态观察黄芪多糖对裸鼠卵巢癌细胞休眠的维持作用  

Astragalus Polysaccharide Has Good Effect on Maintaining Dormancy of Ovarian Cancer Cells in Nude Mice by Living Imaging System

作　　者：胡向丹[1] 王爱爱[1] 翟秋丽 肖静[1] 

机构地区：[1]广东省中医院大学城医院妇科,广东广州510006 [2]广州中医药大学,广东广州510006

出　　处：《新中医》2017年第8期4-7,共4页New Chinese Medicine

基　　金：国家自然科学基金项目(81403422)

摘　　要：目的:利用活体成像技术观察黄芪多糖对裸鼠卵巢癌细胞休眠的维持作用。方法:选取建立成功的卵巢癌细胞休眠裸鼠模型30只随机分成3组:即对照组、黄芪多糖组、顺铂组,每组10只,连续给药14天;利用活体成像系统分别在给药前1天、给药后第7天、第14天监测各组裸鼠卵巢癌细胞休眠情况。结果:与对照组比较,黄芪多糖组给药后第7天、第14天,裸鼠体质量无明显变化;而顺铂组在给药后第7天、第14天,体质量明显降低,差异均有统计学意义(P<0.01)。对照组裸鼠卵巢癌休眠细胞光子量随着时间而升高。与对照组比较,黄芪多糖组和顺铂组在给药第7天、第14天时,裸鼠肿瘤休眠细胞光子量明显降低,差异均有统计学意义(P<0.05,P<0.01)。结论:黄芪多糖具有较好的维持裸鼠卵巢癌细胞休眠的作用,活体成像技术可动态地观察肿瘤细胞休眠的情况,为黄芪多糖应用于临床提供理论依据。Objective： To observe the maintenance effect of Astragalus polysaccharide on the dormancy of ovarian cancer cells in nude mice by living imaging system. Methods： Thirty dormant nude model mice which was successfully established were randomly divided into three groups： the control group, astragalus polysaccharide group, cisplatin group, 10 cases in each. Continuous administration lasted for 14 days; detected dormancy of ovarian cancer cells in nude mice of each group by living imaging system in one day before medication, seven days and fourteen days after medication. Results： Compared with the control group, there was no significant change in the body weight of Astragalus polysaccharide group seven days and fourteenth days after medication, while body weight of Cisplatin group was decreased obviously（P〈 0.01）. Photon amount in the dormant ovarian cancer cells in nude mice of the control group was increased over time. Compared with the control group, photon amount in the dormant ovarian cancer cells in nude mice of astragalus polysaccharide group and cisplatin group was decreased significantly seven days and fourteenth days after medication （P 〈0.05, P 〈0.01）. Conclusion： Astragalus polysaccharide has a good effect on maintaining the dormancy of ovarian cancer cells in nude mice. Living imaging technology can be used to observe the dormancy of tumor cells dynamically, so as to provide a theoretical basis for clinical application of Astragalus Polysaccharide.

关 键 词：黄芪多糖 卵巢癌 人卵巢癌SKOV3细胞 休眠 活体成像 维持作用 动物实验 裸鼠 

分 类 号：R737.31[医药卫生—肿瘤] R285.5[医药卫生—临床医学]