SOCS-1和SOCS-3在慢性根尖周炎病损组织中的表达及临床意义  

Expression of SOCS-1 and SOCS-3 in chronic periapical lesions and its clinical significance

作　　者：王丝墨[1] 马楠[1] 仇丽鸿[1] 李晓琳[1] 杨谛[1] 薛明[1] 

机构地区：[1]中国医科大学口腔医学院牙体牙髓病科辽宁省口腔医学研究所牙体牙髓病学研究室,辽宁沈阳110002

出　　处：《上海口腔医学》2017年第4期384-388,共5页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(2015-A-0000000081500843);沈阳市科技计划项目(F15-199-1-56)

摘　　要：目的:检测细胞因子信号转导抑制因子1(suppressor of cytokine signaling-1,SOCS-1)和细胞因子信号转导抑制因子3(suppressor of cytokine signaling-3,SOCS-3)在慢性根尖周炎病损组织中的表达水平,探讨SOCS-1、3在慢性根尖周炎发病机制中的作用。方法:收集25例慢性根尖周炎病损组织作为病例组,16例健康牙的牙周膜组织作为对照组。对样本中的蛋白表达量采用免疫组织化学法检测,mRNA表达量采用实时定量PCR法检测。采用SPSS13.0软件包对数据进行统计学分析。结果:免疫组织化学结果显示,SOCS-1、3蛋白在慢性根尖周炎病损组织中的表达水平均显著高于对照组(P<0.01);重度炎症组中,SOCS-1、3的蛋白表达显著高于轻中度炎症组(P<0.01)。SOCS-1mRNA在轻中度炎症组、重度炎症组和对照组中的表达量分别为2.620±1.552、2.373±1.083和1.277±1.040,SOCS-3mRNA的表达量分别为9.308±5.901,7.565±3.233和1.232±1.099。SOCS-1、3 mRNA在轻中度炎症组、重度炎症组均显著高于对照组(P<0.01),但不同炎症组之间并无显著差异。结论:SOCS-1、3可能与慢性根尖周炎密切相关。PURPOSE： To detect the expression of suppressor of cytokine signaling-1 （SOCS-1） and suppressor of cytokine signaling-3 （SOCS-3） in chronic periapical lesions and to clarify their roles in pathogenesis of apical periodontitis. METHODS： A total of 25 chronic periapical lesion tissues and 16 normal periodontal ligament tissues were collected respectively. The expression of mRNA was measured by real-time PCR, the protein expression was measured by immunohistochemieal analysis. Statistical analysis was performed using SPSS 13.0 software package. RESULTS： lmmunohistochemical results indicated that expression levels of SOCS-1 and SOCS-3 protein in chronic periapical lesions were significantly higher than that in normal tissues （P〈0.01）; Moreover, SOCS-1 and SOCS-3 protein expression levels in severe inflammation group were significantly higher than that in mild inflammation group （P〈0.01）. In mild inflammation group, severe inflammation group and control group, the expression levels of SOCS-1 mRNA were 2.620±1.552, 2.373± 1.083 and 1.277±1.040, whereas those of SOCS-3 were 9.308 ±5.901, 7.565 ±3.233 and 1.232±1.099, respectively. CONCLUSIONS： The expression levels of SOCS-1 mRNA and SOCS-3 mRNA are significantly higher both in mild inflammation group and severe inflammation group than that in control group （P〈0.01）, while no significant difference is found in mild and severe inflammation group.

关 键 词：慢性根尖周炎 细胞因子信号转导抑制因子1 细胞因子信号转导抑制因子3 实时荧光定量PCR 

分 类 号：R781.34[医药卫生—口腔医学]