冬虫夏草人工繁育品与野生品基于甾醇特征图谱的比较研究  

Comparative Study of Characteristic Chromatogram of Cultivated and Wild Cordyceps sinensis Based on Sterols

作　　者：过立农[1] 张美 刘杰[1] 马双成[1] 钱正明 李文佳 昝珂[1] 郑健[1] 

机构地区：[1]中国食品药品检定研究院,北京100050 [2]宁夏回族自治区食品药品检验所,银川750001 [3]广东东阳光药业有限公司,东莞523808

出　　处：《中国药事》2017年第8期951-959,共9页Chinese Pharmaceutical Affairs

基　　金：中医药行业科研专项"中药饮片质量保障系统研究(一)"资助(编号201507002);十二五国家科技重大专项(编号2014ZX09304307-002)

摘　　要：目的:对冬虫夏草人工繁育品与野生品进行比较研究,建立基于甾醇类成分的特征图谱,并比较麦角甾醇含量的差异,为该药材的质量监管提供参考。方法:以10批冬虫夏草人工繁育品、17批野生品以及5批凉山虫草为研究对象,采用超高效液相色谱法,以麦角甾醇为对照品,选用XBridge C18色谱柱(100 mm×2.1 mm,1.6μm);流动相为甲醇-水(95∶5),检测波长282 nm,流速0.3 m L·min^(-1),柱温30℃,采用CHEMPATTERN化学计量学软件对野生品色谱图进行处理,生成特征图谱,并计算所有批次的相似度。结果:17批野生冬虫夏草、10批冬虫夏草人工繁育品与野生品对照图谱之间的相似度均大于0.9,相似度良好;而5批凉山虫草相似度均未超过0.8,相似度较低。10批人工繁育品的平均含量为0.121%±0.031%;17批野生品的平均含量为0.115%±0.045%;5批凉山虫草的平均含量为0.468%±0.038%。冬虫夏草人工繁育品和野生品基于甾醇的特征图谱以及麦角甾醇含量基本一致;与凉山虫草有显著性差异。结论:本研究有助于冬虫夏草的真伪鉴别,同时为冬虫夏草人工繁育资源开发以及合理使用提供科学依据。Objective： To establish the UPLC characteristic, chromatogram based on sterols and to compare the differences of the contents of ergosterol in cultivated and wild Cordycepssinensis so as to provide references for the supervision of this crude drag, Methods： 10 batches of cultivated Cordyceps sinensis, 17 batches of wild Cordyceps sinensis and 5 batches of Cordyceps liangshanensis were used as objects and ergosterol was used as reference substance. UPLC analysis was performed on a Waters XBridge C 18 column （100×2.1 mm; 1.6 μm）. The mobile phase was methanol-water （95：5） with isocratic elution. The flow rate was 0.3 mL·min^-1 and the detection wavelength was 282 nm and the column temperature was 30℃. Chemometrics software Chempattern was employed to generate characteristic chromatogram and calculate similarity. Results： Similarities of 17 batches of wild Cordyceps sinensis and 10 batches of cultivated Cordyceps sinensis were more than 0.9, indicating good similarity. But the similarities of 5 batches of Cordyceps liangshanensis were no more than 0.8, indicating poor similarity. The average contents of ergosterol were 0.121% ± 0.031% in cultivated Cordyceps sinensis, 0.115% ± 0.045% in wild Cordyceps sinensis, and 0.468% ± 0.038% in Cordyceps liangshanensis. UPLC characteristic chromatogram and content of ergosterol in cultivated Cordyceps sinensis were basically identical to those of the wild Cordyceps sinensis and showed significant differences from adulterant Cordyceps liangshanensis. Conclusion： The results were helpful to authenticate Cordyceps sinensis and could provide scientific references for the utilization of cultivated Cordyceps sinensis, and the resource development of chinese crude dyug.

关 键 词：冬虫夏草繁育品 野生冬虫夏草 凉山虫草 特征图谱 甾醇 麦角甾醇 含量比较 中药材资源开发 

分 类 号：R917[医药卫生—药物分析学] R284.1[医药卫生—药学]