TALEN靶向敲除REST对U-87细胞增殖和迁移的影响  

The effect of REST knockout by TALEN on proliferation and migration of U-87 cells

作　　者：张殿宝[1] 李莹[1] 林学文[1] 王瑞[1] 阚周密[2] 庞希宁[1,3] 

机构地区：[1]中国医科大学基础医学院干细胞与再生医学研究室,沈阳110122 [2]中国医科大学第一临床学院药剂科,沈阳110122 [3]中国医科大学科学实验中心,沈阳110122

出　　处：《解剖科学进展》2017年第4期337-339,共3页Progress of Anatomical Sciences

基　　金：国家重点基础研究发展计划(2012CB518103);中国医科大学校内科研基金(XZR20160022);沈阳市干细胞与再生医学重点实验室项目(F15-157-1-00)

摘　　要：目的研究TALEN(transcription activator-like effector nuclease)靶向敲除REST(repressor element 1-silencing transcription factor)对U-87细胞增殖和迁移能力的影响。方法使用分子克隆手段构建TALEN质粒;通过western blot分析TALEN打靶效率;分别使用CCk-8法和Transwell小室法检测细胞增殖和迁移能力。结果 TALEN能够有效打靶U-87细胞中的REST,蛋白水平显著下调;TALEN靶向敲除REST能够显著降低U-87细胞的增殖和迁移水平。结论 TALEN能够高效打靶U-87细胞中的REST,进而抑制U-87细胞的增殖和迁移能力。Objective To study the effects of REST （repressor element 1-silencing transcription factor） knockout by TALEN （transcription activator-like effector nuclease） on cell proliferation and migration in U-87 cells. Methods The TALEN plasmids were constructed using molecular cloning methods. The genome targeting efficiency of TALEN was assessed by western blot. The cell proliferation and migration were analyzed by CCK-8 assay and Transwell assay separately. Results REST was targeted effectively by TALEN in U-87 cells and REST protein level was significantly reduced. The proliferation and migration of U-87 cells were significantly reduced upon REST knockout by TALEN. Conclusion REST knocked down by TALEN, inhibited the proliferation and migration of U-87 cells.

关 键 词：TALEN REST 神经胶质瘤 增殖 迁移 

分 类 号：R739.41[医药卫生—肿瘤]