CAK复合体在雌激素受体阳性乳腺癌中的表达及其临床意义  

Expression and clinical significance of CAK complex in estrogen receptor-positive breast cancer

作　　者：李丹[1] 胡海峰[1] 夏秀林[1] 

机构地区：[1]陕西省宝鸡市妇幼保健院乳腺科,721000

出　　处：《国际肿瘤学杂志》2017年第7期500-503,共4页Journal of International Oncology

摘　　要：目的 探究细胞周期蛋白依赖性激酶活化激酶（CAK）复合体各组分细胞周期蛋白依赖性激酶7（CDK7）、周期素H和辅助蛋白MAT1在雌激素受体阳性乳腺癌中的表达及其临床意义.方法选取在陕西省宝鸡市妇幼保健院乳腺科诊治的40例雌激素受体阳性乳腺癌患者,收集患者手术的乳腺癌组织和癌旁正常组织.实时荧光定量PCR（qRT-PCR）检测组织中CDK7、周期素H、MAT1的表达情况,分析其与患者临床病理特征的相关性.结果 CDK7、周期素H和MAT1在肿瘤组织中的相对表达水平分别为2.54±0.78、2.21±0.56、2.46±0.58,在癌旁正常组织的相对表达水平分别为1.26±0.30、1.16±0.42、1.17±0.39,差异均有统计学意义（t=9.654,P〈0.001;t=9.433,P〈0.001;t=11.741,P〈0.001）;周期素H和MAT1表达水平越高,临床分期越低,具有明显的相关性（U=3.17,P=0.01;U=2.53,P=0.01）;此外,MAT1水平高的患者肿瘤偏小（χ2=14.16,P=0.01）.结论 CAK复合体CDK7、周期素H和MAT1在雌激素受体阳性乳腺癌患者肿瘤组织中高表达,周期素H和MAT1与临床分期密切相关,且MAT1与患者肿瘤大小也具有明显相关性.Objective To evaluate the expression and clinical significance of cyclin dependent kinase （CDK）-activating kinase （CAK） complex including CDK7, cyclin H and accessory protein menage a trios 1 （MAT1） in estrogen receptor-positive breast cancer.Methods A total of 40 patients with estrogen receptor-positive breast cancer from Department of Galactophore, Baoji Maternal and Child Care Service Centre of Shaanxi Province were investigated in this study.Breast cancer tissues and adjacent normal tissues were obtained from patients undergoing surgery.The mRNA expressions of CDK7, cyclin H and MAT1 in two types of tissues were measured by real-time fluorescent quantitative （qRT）-PCR, and their correlations with clinicopathologic features of patients were analyzed.Results The expressions of CDK7, cyclin H and MAT1 in cancer tissues were 2.54±0.78, 2.21±0.56 and 2.46±0.58, while those in adjacent normal tissues were 1.26±0.30, 1.16±0.42 and 1.17±0.39, and there were significantly differences between different types of tissues （t=9.654, P〈0.001;t=9.433, P〈0.001;t=11.741, P〈0.001）.The higher expressions of cyclin H and MAT1 in patients′ cancer tissues had the lower clinical stage, with significant correlations （U=3.17, P=0.01;U=2.53, P=0.01）.In addition, the tumors were smaller in patients with higher expression levels of MAT1 （χ2=14.16, P=0.01）.Conclusion The expressions of CAK complex CDK7, cyclin H and MAT1 are elevated in estrogen receptor-positive breast cancer patients.Cyclin H and MAT1 are closely associated with clinical grade, and MAT1 is also significantly associated with tumor size.

关 键 词：乳腺肿瘤 诊断 CAK复合体 

分 类 号：R737.9[医药卫生—肿瘤]