HSP90抑制剂17-DMAG对急性淋巴细胞白血病Jurkat细胞株增殖和凋亡的影响  

Effect of Heat Shock Protein 90 Inhibitor 17-DMAG on Proliferation and Apoptosis of Acute Lymphocytic Leukemia Cell Line Jurkat

作　　者：葛芳芳[1] 郭荣[1] 田文亮[1] 高风彩[1] 孙玲[1] 姜中兴[1] 

机构地区：[1]郑州大学第一附属医院血液科,河南郑州450052

出　　处：《中国实验血液学杂志》2017年第4期1011-1015,共5页Journal of Experimental Hematology

摘　　要：目的:探讨热休克蛋白90(HSP90)抑制剂17-二甲胺乙胺基-17去甲氧基格尔德霉素(17-DMAG)对急性淋巴细胞白血病Jurkat细胞的增殖及凋亡的影响。方法:收集Jurkat细胞,应用HSP90抑制剂17-DMAG作用于细胞株,通过半定量PCR检测HSP90的基因表达,WST技术检测17-DMAG对细胞增殖的影响,Annexin V/PI双染流式细胞术检测细胞凋亡。结果:不同浓度17-DMAG作用于Jurkat细胞48 h后,HSP90 mRNA表达明显减少,且呈剂量依赖性(r=0.9530,P<0.01);17-DMAG处理Jurkat细胞48 h的IC50为3.17μmol/L,作用于Jurkat细胞后抑制Jurkat细胞增殖(r=0.9903,P<0.01),促进Jurkat细胞凋亡,且呈剂量依赖性(r=0.9876,P<0.01)。结论:HSP90抑制剂17-DMAG能够抑制急性淋巴细胞白血病Jurkat细胞株的增殖并诱导其凋亡。Objective： To explore the effect of heat shock protein 90（ HSP90） inhibitor 17-DMAG,an inhibitor specific for heat shock protein 90,on the proliferation and apoptosis of acute lymphocytic leukemia cell lines Jurkat.Methods： Jurkat cells were collected,then were treated with 17-DMAG. The expression of HSP90 was examined by semi-quantitative RT-PCR analysis,the effect of 17-DMAG on cell proliferation were detected by using WST,and cell apoptosis were detected by using flow cytometry with Annexin V/PI double stenining. Results： After Jurkat cells were treated with different concentrations of 17-DMAG for 48 hours,the HSP90 mRNA expression decreased significantly in dose dependent manner（ r = 0. 9530,P〈0. 01）. The IC50 was 3. 17 mmol/L when the Jurkat cells were treated with 17-DMAG for 48 h; after treating Jurkat cell with 17-DMAG,the cell proliferation was inhibited（ r = 0. 9903,P〈0. 01）,the cell apoptosis was increased in dose dependent manner（ r = 0. 9876,P〈0. 01）. Conclusion： 17-DMAG can inhibit the Jurkat cell proliferation and induce the Jurkat cell apoptosis.

关 键 词：急性淋巴细胞白血病 热休克蛋白90 17-二甲胺乙胺基-17去甲氧基格尔德霉素 JURKAT细胞 

分 类 号：R733.71[医药卫生—肿瘤]