P120ctn基因沉默促进舌癌细胞上皮-间质转化  

P120ctn silencing can promote the epithelial-mesenchymal transition of tongue squamous cancer cell

作　　者：潘夏薇[1] 陈钟[1] 陈江[2] PAN Xiawei CHEN Zhong CHEN Jang(Department of Stoma tology, Xiamen Medical College, Xiamen, Fujian 362008, China Department of Stomatology ,Fujian Medical University ,Fuzhou,Fujian 350108 ,China)

机构地区：[1]厦门医学高等专科学校口腔医学系,福建厦门361008 [2]福建医科大学口腔医学系,福州350108

出　　处：《检验医学与临床》2017年第16期2352-2354,2358,共4页Laboratory Medicine and Clinic

基　　金：厦门医学院自然科学基金资助项目(Z2013-02)

摘　　要：目的探索P120-连环蛋白(P120ctn)在口腔鳞状细胞癌(OSCC)上皮-间质转化(EMT)中的作用。方法采用质粒pGFP-V-RS-P120ctn shRNA转染人舌鳞癌细胞(TSCCA),采用实时荧光定量聚合酶链反应、Western blot检测细胞中P120ctn、E-钙黏蛋白(E-cad)、N-钙黏蛋白(N-cad)和波形蛋白(Vim)的mRNA和蛋白表达的变化,通过Transwell细胞侵袭及细胞迁移试验检测转染前、后细胞迁移和侵袭能力的变化。结果用质粒pGFP-V-RS-P120ctn shRNA转染TSCCA后发现,随着P120ctn表达的明显降低,E-cad的mRNA和蛋白表达水平明显降低,N-cad和Vim的mRNA和蛋白表达水平明显升高,细胞迁移和侵袭能力明显提高,差异均有统计学意义(P<0.05)。结论在OSCC中P120ctn可能通过调控EMT标记物E-cad的表达参与EMT的发生,调节肿瘤的侵袭和转移。Objective To investigate the role of P120-catenin（P120ctn）in the process of the epithelial-mesenchymal transition（EMT）of oral squamous-cell cancer（OSCC）.Methods Plasmid pGFP-V-RS-P120 ctn shRNA was used to transfect TSCCA cells.Real-time fluorescent quantitative PCR and Western blot were adopted to test mRNA and protein expressions of P120 ctn,E-cadherin（E-cad）,N-cadherin（N-cad）and Vimentin（Vim）,and Transwell cell invasion and cell migration assay were used to test the invasion and migration capacity before and after the transfection.Results After plasmid pGFP-V-RS-P120 ctn shRNA was used to transfect TSCCA cells,we found that as the expression of P120 ctn significantly decreased,mRNA and protein expression of E-cad decreased significantly,mRNA and protein expression of N-cad and Vim increased significantly,and cell migration and invasion capacity increased significantly（P〈0.05）.Conclusion In OSCC cells,P120 ctn may be involved in EMT and the metastasis and invasion regulation by adjusting EMT biomarker E-cad expression.

关 键 词：P120-连环蛋白 上皮-间质转化 口腔 鳞状细胞癌 

分 类 号：R730.2[医药卫生—肿瘤]