miR-101在胰腺癌组织中的表达水平及对癌细胞侵袭能力的影响  

Expression and Effect of miR-101 on Invasion in Patients with Pancreatic Cancer

作　　者：李晓怡 陈小丽[1] 张琴[1] 刘水逸[1] 石玉香[2] 卢忠心[1] 陈卫群[3] LI Xiao-yi CHEN Xiao-li Zhang Qin LIU Shui-yi SHI Yu-xiang LU Zhong-xin CHEN Wei-qun(Department of Clinical Laboratory The Central Laboratory,3Department of Pathology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Tech- nology, Wuhan 430014, China)

机构地区：[1]华中科技大学同济医学院附属武汉中心医院检验科,武汉430014 [2]华中科技大学同济医学院附属武汉中心医院病理科,武汉430014 [3]华中科技大学同济医学院附属武汉中心医院中心实验室,武汉430014

出　　处：《微循环学杂志》2017年第3期11-15,共5页Chinese Journal of Microcirculation

基　　金：湖北省自然科学基金重点项目(2015CFA078);武汉市卫计委科研项目(WX14C13;WX14B10)

摘　　要：目的:检测分析胰腺癌组织和胰腺癌细胞株中微小RNA(miRNA)的表达情况及对胰腺癌细胞侵袭能力的影响。方法:收集临床患者胰腺癌组织、癌旁组织标本及4种典藏胰腺癌细胞株,用实时荧光定量聚合酶链反应(qRT-PCR)检测所有样本中miR-101的表达水平;采用Transwell侵袭试验检测miR-101对胰腺癌细胞株PANC-1和MIA PaCa-2的侵袭能力,同时采用Western Blotting检测转染miR-101的模拟物(mimic)后胰腺癌细胞株MIA PaCa-2的Rho相关卷曲螺旋蛋白激酶2(Rhoassociated coiled coil forming protein kinas,ROCK2)蛋白表达水平。结果:4种胰腺癌细胞株中miR-101表达量较正常胰腺细胞明显降低(P<0.05),胰腺癌组织中miR-101表达量也明显低于癌旁组织(P<0.05)。转染组miR-101的胰腺癌细胞株PANC-1和MIA PaCa-2的侵袭能力较未转染组明显减弱(P<0.05),转染组胰腺癌细胞株MIA PaCa-2中ROCK2蛋白表达也显著低于未转染组(P<0.05)。结论:胰腺癌组织和胰腺癌细胞株均低表达miR-101,可能靶向抑制ROCK2蛋白,减弱胰腺癌细胞的侵袭能力。Objective：To investigate the expression of microRNA in pancreatic cancer tissue of patients and pancreatic cancer cell lines and the effect on invasion of pancreatic cancer.Method：Constitutive expression of miR-101 was detected by quantitative real-time polymerase chain reaction（qRT-PCR）in pancreatic cancer tissue and adjacent tissue,the relative expression of miR-101 was measured by qRT-PCR in 4pancreatic cancer cells or normal pancreatic cell.The protein level of target gene ROCK2 was examined by western blot and the ability of cell invasion was detected by transwell after transfection of miR-101 in MIA PaCa-2.Results：The expression of miR-101 was downregulated in pancreatic cancer tissue and 4pancreatic cancer cells（P〈0.05）.After overexpressing miR-101 in MIA PaCa-2and PANC-1,the protein level of target gene-ROCK2 was reduced and the ability of pancreatic cell invasion was also inhibited（P〈0.05）.Conclusion：miR-101 was downregulated in pancreatic cancer tissue and 4pancreatic cancer cells,miR-101 may have a great effect on pancreatic cancer cell invasion by directly target ROCK2.

关 键 词：微小RNA-101 胰腺癌 侵袭 Rho相关卷曲螺旋蛋白激酶2 

分 类 号：R735.9[医药卫生—肿瘤]