淡紫紫孢菌防治采后番茄果实灰霉病  

Efficacy of Purpureocillium lilacinus on Gray Mold of Post-harvest Tomato Fruits

作　　者：袁和奇 徐鹏程[1] 肖海艳[1] 张臻[1] 赵娟[1] 王高峰[1] 肖雪琼[1] 肖炎农[1] 

机构地区：[1]华中农业大学植物科学技术学院/湖北省植物病害监测与安全控制重点实验室,武汉430070

出　　处：《中国生物防治学报》2017年第4期525-530,共6页Chinese Journal of Biological Control

基　　金：国家公益性行业(农业)科研专项(201303025)

摘　　要：本文通过孢子萌发检测、平板生长抑制法和果实病害防治试验,测定了淡紫紫孢菌Purpureocillium lilacinus PL36-1防治储藏期番茄果实灰霉病的效果。结果表明,菌株PL36-1发酵滤液不仅能够抑制灰葡萄孢分生孢子萌发和芽管伸长,而且可以抑制灰葡萄孢菌丝生长。对峙培养的菌株PL36-1显著抑制灰葡萄孢的菌丝生长,菌丝生长抑制率可达到74.45%,菌株PL36-1产生的挥发性物质也对灰葡萄孢菌丝生长有一定的抑制作用。菌株PL36-1发酵液处理樱桃番茄果实后,能有效控制番茄果实灰霉病的发生,含有2×10~8孢子/m L的发酵液10 d的防治效果达70.64%。The effect of Purpureocillium lilacinus PL36-1 on gray mold of post-harvest tomato fruits was evaluated. The results showed that the fermentation filtrate of strain PL36-1 inhibited mycelial growth of Botrytis cinerea, as well as conidial germination and elongation of germ tubes. Mycelial growth of B. cinerea decreased by 74.45% when it was dual cultured with the strain PL36-1 in petri dish. The VOCs from PL36-1 also suppressed the mycelial growth of B. cinerea. The PL36-1 could control post-harvest tomato fruits gray mold effectively, the incidence of the disease fell by 70.64% after 10 days treated by PL36-1 fermentation broth with 2×10^8 spores/m L. It was suggested that P. lilacinus was potential to be used as a biocontrol agent in management of post-harvest tomato fruits gray mold.

关 键 词：淡紫紫孢菌 番茄灰霉病 采后病害 抑菌作用 

分 类 号：S476[农业科学—农业昆虫与害虫防治]