HPLC切换波长法同时测定舒肝健胃丸中五种活性成分  

Simultaneous Determination of Five Components in Shugan Jianwei Pills by Switching Wavelength of HPLC

作　　者：孙艳涛[1,2] 邓欣宇 付瑶[1,2] 赵磊[3] 孙鑫[1,2] 张原旗 于浪天 冯静东[1,2] SUN Yan-tao DENG Xin-yu FU Yao ZHAO Lei SUN Xin ZHANG Yuan-qi YU Lang-tian FENG Jing-dong(College of Chemistry, Jilin Normal University, Siping 136000, China Key Laboratory of Preparation and Applications of Environmmental Frendly Materials,Jilin Normal University, Ministry of Education China,Siping 136000 ,China Siping Institute Food and Drug Control, Siping 136000, China College of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing 211198, China)

机构地区：[1]吉林师范大学化学学院,吉林四平136000 [2]吉林师范大学环境友好材料制备与应用教育部重点实验室,吉林四平136000 [3]吉林省四平市食品药品检验所,吉林四平136000 [4]中国药科大学中药学院,江苏南京211198

出　　处：《吉林师范大学学报（自然科学版）》2017年第3期74-78,共5页Journal of Jilin Normal University:Natural Science Edition

基　　金：国家级大学生创新创业训练计划项目(2017);吉林省教育厅项目(JJKH20170373KJ);四平市科技局项目(2014042;2016048)

摘　　要：建立HPLC法同时测定舒肝健胃丸中五种成分(芍药苷、柚皮苷、橙皮苷、和厚朴酚与厚朴酚)含量的方法.采用高效液相色谱法,色谱柱为TC-C18(4.6 mm×250 mm,5μm),检测波长为0~10 min,230 nm,10~20 min,283 nm和20~55 min,294 nm;流动相为乙腈-水溶液,梯度洗脱0~15 min,22%~22%;15~25 min,22%~72%;25~35 min,72%~72%;35~40 min,72%~90%;40~50 min,90%~90%;50~55 min,90%~22%;流速为1.0 m L·min-1;柱温为25℃.芍药苷、柚皮苷、橙皮苷、和厚朴酚与厚朴酚的质量浓度分别在0.672~67.2 mg·mL^(-1)、3.124~312.4 mg·mL^(-1)、2.3712~237.12 mg·mL^(-1)、0.44~440 mg·mL^(-1)和1.196-1~119.6 mg·mL^(-1)范围内呈良好的线性关系.测定舒肝健胃丸中芍药苷、柚皮苷、橙皮苷、和厚朴酚和厚朴酚的含量分别为0.68 mg·g^(-1)、2.85 mg·g^(-1)、6.75 mg·g^(-1)、1.09 mg·g^(-1)和1.09 mg·g^(-1).本方法简单方便、准确可靠、专属性强,为舒肝健胃丸药品的质量控制提供了方法依据.To establish an HPLC method for simultaneously determining five components such as paeoniflorin, naringin, hesperidin, magnolol and honokiol in Shugan Jianwei pills by HPLC. An RP-HPLC method was established with TC-C18 （4.6 mm×250 mm,5 μm）column. Detection wavelengths were set at 230 nm between 0 and 10 min,283 nm between 10 and 20 min,and 294 nm between 20 and 55 min,respectively. The mobile phase was acetonitrile-water with a gradient program （0 -15 min,22% -22% ;15 -25 min,22% -72% ;25 - 35 rain ,72% - 72% ;35 -40 min ,72% - 90% ;40 - 50 min ,90% - 90% ;50 - 55 min,90% - 22% ）. The flow rate was 1 mL · min-1. The column temperature was 25 ℃. There was a good linearity between the absorption peak area and the concentration for paeoniflorin, naringin, hesperidin, magnolol and honokiol in the ranges of 0. 672 -67.2 mg · mL^-1 ,3. 124 -312.4 mg · mL^-1 ,2. 3712 -237.12 mg· mL-I ,0.44 -440 mg·mL^-1 and 1. 196- 119. 6 mg · mL^-1, respectively. The contents of paeoniflorin, naringin, hesperidin, magnolol and honokiol in Shugan Jianwei pills were 0.68 mg·g^-1,2.85 mg · g^-1,6.75 mg·g^-1,1.09 mg·g^-1 and 1.09 mg ·g^- 1 ） respectively. The method is accurate, sensitive, reliable, and with good reproducibility, which provides a reference for the quality control of Shugan Jianwei pills.

关 键 词：舒肝健胃丸 高效液相色谱法 含量测定 

分 类 号：O657.7[理学—分析化学]