紫草宁对乳腺癌细胞的作用  

Shikonin inhibits the proliferation of breast cancer cell lines in vitro and in vivo

作　　者：陆奕含 屠俊浩 张林鑫[1] 张谌翰 徐露[3] 王珏[3] 凌立君[3] 

机构地区：[1]南京医科大学,210029 [2]苏州市吴中区人民医院,215000 [3]南京医科大学第一附属医院乳腺外科,210029

出　　处：《中华实验外科杂志》2017年第8期1313-1315,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 观察紫草宁对乳腺癌细胞的作用.方法 采用4种乳腺癌细胞株,分为对照组和用药组进行多项体外实验,观察紫草宁对乳腺癌细胞株的增殖、凋亡、周期的影响.同时于小鼠体内建立移植瘤模型,对比对照组与用药组肿瘤体积增长的速度.结果 经不同浓度的紫草宁处理乳腺癌细胞株后,得出MCF7、ZR-75-1、SUM1315和MDA-MB-231的半数抑制浓度（IC50）值分别为（1.165±0.025）、（1.043±0.010）、（1.595±0.006）、（1.516±0.015）μmol/L.对应IC50浓度紫草宁诱导乳腺癌细胞凋亡后,与阴性对照组比较,药物处理组的细胞凋亡率显著增高（MCF7：P=0.001;ZR-75-1：P=0.001;SUM1315：P=0.001;MDA-MB-231：P=0.001）.同时,紫草宁处理组的G1期细胞分数显著增加,提示紫草宁具有阻滞细胞周期于G1期的作用.在体内实验中,紫草宁显著抑制了SUM1315细胞建立的小鼠移植瘤模型的肿瘤增长速度,且药物高浓度组与低浓度组之间差异亦有统计学意义（P=0.000）.结论 紫草宁可于体内及体外抑制乳腺癌细胞的增殖.Objective To further examine the effects of shikonin on breast cancer cells.MethodsFour typical breast cancer cell lines were used.The effects of shikonin were evaluated through a series of experiments in vitro and in vivo.Results The cell counting kit-8 （CCK-8） assay revealed shikonin inhibited proliferation of MCF7,ZR-75-1,SUM1315 and MDA-MB-231 breast cancer cells in a dose-dependent manner.The 50% inhibitory dose （IC50） of MCF7,ZR-75-1,SUM1315 and MDA-MB-231 were 1.165±0.025,1.043±0.010,1.595±0.006 and 1.516±0.015 μmol/L,respectively.Flow cytometry demonstrated shikonin promoted cell cycle arrest in the G1 phase and induced cell apoptosis.The apoptosis rate of MCF7,ZR-75-1,SUM1315 and MDA-MB-231 were 12.1±0.7 （P=0.001）,9.1±0.4 （P=0.001）,15.0±0.9 （P=0.001）,15.3±1.3 （P=0.001）,respectively.When compared with control group,the percentages of G1 phase were 15.8±0.7 （P=0.019）,32.3±2.0 （P=0.038）,45.0±2.5 （P=0.010）,15.8±0.4 （P=0.001）,respectively.Moreover,shikonin significantly reduced the growth of tumor xenografts derived from SUM1315 breast cancer cells in nude mice.And the efficacy was associated with shikonin concentrations.A statistical difference was found between Group C and A on the 7th day （P=0.047）.On the 9th day,there was statistical difference between Group C and A（P=0.011）,so as Group C and B （P=0.005）.On the 11th day,the comparison between any two groups have statistical significance （A vs.B： P=0.046;B vs.C： P=0.024;A vs.C： P=0.007）.Conclusion The study demonstrates shikonin can inhibit the proliferation of breast cancer cell lines in vitro and in vivo.

关 键 词：紫草宁 乳腺癌 增殖 

分 类 号：R737.9[医药卫生—肿瘤]