HOX转录反义RNA在三阴性乳腺癌组织的表达及意义  

Expression of HOX transcription antisense RNA in triple-negative breast cancer and its clinical significance

作　　者：程苒[1] 崔军威[1] 方征宇[2] 刘晓岭[1] 韦伟[1] 

机构地区：[1]北京大学深圳医院乳腺外科,深圳518036 [2]深圳北京大学香港科技大学医学中心,518036

出　　处：《中华实验外科杂志》2017年第8期1391-1393,共3页Chinese Journal of Experimental Surgery

基　　金：深圳市科技项目（JCYJ20130402114702122）

摘　　要：目的 检测三阴性乳腺癌（TNBC）中长链非编码RNA HOX转录反义RNA（HOTAIR）的相对表达,探讨其与乳腺癌恶性程度及进展的关系.方法 选取TNBC患者组织样本43例,非TNBC患者组织样本78例（均留取癌灶及癌旁正常乳腺组织）,采用实时荧光定量聚合酶链反应（Real-time PCR）的方法检测癌灶及其配对癌旁正常乳腺组织中HOTAIR的相对含量,比较其表达差异;检测TNBC与非TNBC癌灶中HOTAIR的相对含量,比较其表达差异;同时分析TNBC癌灶中HOTAIR的相对含量与肿瘤临床病理分期的关系.结果 TNBC和非TNBC组织样本中,癌灶与癌旁组织比较HOTAIR的相对表达显著升高（0.59±0.50比0.41±0.32,P=0.001）;HOTAIR的相对表达在TNBC中较非TNBC中高（0.78±0.59比0.48±0.41,P=0.004）;TNBC样本中,晚期样本较早中期样本HOTAIR相对表达水平升高（1.03±0.63比0.54±0.44,P=0.006）.结论 HOTAIR在癌灶中表达TNBC较非TNBC明显升高,与TNBC的临床病理分期明显相关.Objective To explore the HOX transcription antisense RNA （HOTAIR） relative expression in triple-negative breast cancer （TNBC） and its correlation with malignancy and progression of breast cancer.Methods Relative expression levels of HOTAIR were examined by quantitative reverse transcription-polymerase chain reaction （Real-time PCR） in 43 TNBC patients and 78 non-TNBC patients.The relative expression of HOTAIR was compared between carcinoma tissues and adjacent normal tissues （ANTs）.The correlation between the relative expression of HOTAIR and the cancer stages of the TNBC patients was analyzed.Results We found that the relative expression levels of HOTAIR were signifiantly increased in breast cancer tissues as compared with that in ANTs （0.59±0.50 vs.0.41±0.32,P=0.001）.The relative expression of HOTAIR was more enhanced in TNBC than in non-TNBC （0.78±0.59 vs.0.48±0.41,P=0.004）.Higher relative expression levels of HOTAIR were found in advanced TNBC than in early-stage TNBC （1.03±0.63 vs.0.54±0.44,P=0.006）.Conclusion Relative expression of HOTAIR was enhanced in TNBCs and might correlate with cancer stages of TNBC.

关 键 词：三阴性乳腺癌 长链非编码RNA HOX转录反义RNA 肿瘤分期 

分 类 号：R737.9[医药卫生—肿瘤]