牙本质内源性聚糖对树脂-牙本质粘接界面耐水储存老化能力的影响  

Effect of dentin proteoglycans on the stability of resin-dentin bonds against artificial saliva storage

作　　者：高宇[1,2] 赵三军[1,3] 王培欢[1,3] 芦帅[1,4] 陈吉华[1] 

机构地区：[1]第四军医大学口腔医学院口腔修复科、军事口腔医学国家重点实验室、口腔疾病国家临床医学研究中心、陕西省口腔医学重点实验室,西安710032 [2]山东大学口腔医学院儿童口腔科、山东省口腔组织再生重点实验室,济南250012 [3]济南军区总医院口腔科,济南250031 [4]成都军区机关医院口腔科,成都610011

出　　处：《中华口腔医学杂志》2017年第8期486-491,共6页Chinese Journal of Stomatology

基　　金：国家自然科学基金（81170985、81470773）

摘　　要：目的 比较分别去除牙本质蛋白聚糖和糖胺聚糖侧链后树脂-牙本质粘接界面的耐水储存老化能力,探索两者在牙本质粘接耐久性中的作用.方法 取第四军医大学口腔医学院口腔颌面外科提供的72颗无龋第三磨牙,制备标准牙本质粘接面,37%磷酸酸蚀15 s后用随机数字表法随机分为3组（每组24颗）,分别使用硫酸软骨素酶ABC（chondroitinase ABC,C-ABC）（C-ABC组,即去除糖胺聚糖）、胰蛋白酶（胰蛋白酶组,即去除蛋白聚糖）和去离子水（阴性对照组）37℃水浴振荡孵育48 h.制备树脂-牙本质粘接试件,各组粘接试件用随机数字表再随机分为3个亚组（每亚组8颗）：即刻亚组、水储存6和12个月亚组,各亚组相应处理后测试微拉伸强度（即粘接强度）,观察断裂模式、界面微观形貌.结果 胰蛋白酶组即刻及水储存6和12个月后的粘接强度[（49.04±3.57）、（37.01±3.21）和（35.27±3.56）MPa]均显著高于阴性对照组[（40.71±3.32）、（28.87±2.34）和（24.20±2.07）MPa]（P＜0.05）;C-ABC组即刻粘接强度[（32.94±2.45）MPa]显著小于阴性对照组（P＜0.05）,水储存6和12个月后粘接强度[（26.46±2.45）和（22.50±2.58）MPa]与阴性对照组差异无统计学意义（P＞0.05）.随老化时间延长,各组粘接界面内聚破坏占比增大.水储存后阴性对照组混合层出现裂隙,其他两组均未见裂隙.结论 去除蛋白聚糖可同时提高牙本质粘接强度和耐久性,去除糖胺聚糖侧链可降低牙本质的粘接强度,但提高了粘接耐久性.Objective To investigate the potential effect of proteoglycans （PG） and glycosaminoglycans （GAG） on the stability of resin-dentin bonds against artificial saliva storage. Methods Seventy-two extracted molars were used to obtain standard dentin bonding surface, and the specimens were etched for 15 s with 37% phosphoric acid and divided into three groups using a table of random number. Then the three groups undergone different incubating procedures as follow： specimens in chondroitinaseABC （C-ABC） group were incubated with C-ABC, specimens in trypsin （TRY） group were incubated with trypsin, and specimens in the control group were incubated with deionized water. All specimens were incubated at 37 ℃ for 48 h in the oscillators. Then specimens in each group were randomly assigned into three subgroups （n=8） as follows： immediate control subgroup, aging subgroups with artificial saliva storage for 6 months and 12 months. Microtensile bond strength （μTBS）, fracture mode, bonding interface morphology and nanoleakage were evaluated. Results Immediately and with artificial saliva storage for 6 months and 12 mouths, the μTBS of TRY group （[49.04±3.57], [37.01±3.21] and [35.27±3.56] MPa） were significantly higher than those in the control group （[40.71±3.32 ], [28.87±2.34] and [24.20±2.07] MPa） （P〈0.05）. The immediate μTBS of C-ABC group （[32.94±2.45] MPa） was significantly lower than that of the control group （P〈0.05）. While with artificial saliva storage for 6 months and 12 mouths, the μTBS of C-ABC group （[26.46±2.45] and [22.50±2.58] MPa） were no differences with those of the control group （P〉0.05）. The ratio of cohesive fracture increased with the extension of aging time. Some narrow gaps were found in hybrid layer of the control group with artificial saliva storage for 6 months and 12 mouths. Conclusions Removal of PG increased the μTBS and durable bonds to dentin, while removal of GAG decreased theμTBS, however, it can be of help to create m

关 键 词：蛋白聚糖类 牙本质粘结剂 拉伸强度 纳米渗漏 

分 类 号：R783.1[医药卫生—口腔医学]