基于转录组分析华细辛甲基丁香酚生物合成途径的相关基因  

Identification of genes related to biosynthesis of methyleugenol in Asarum sieboldii by transcriptome sequence analysis

作　　者：林懋怡 牛卉[1] 刘晋杰[1] 柳威[1] 刘忠[1] 

机构地区：[1]上海交通大学药学院,上海200240

出　　处：《中草药》2017年第15期3160-3167,共8页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金项目(81373962;31570325);上海交通大学医工交叉基金项目(YG2013MS72)

摘　　要：目的获得华细辛Asarum sieboldii转录组数据库和差异表达基因,识别华细辛甲基丁香酚生物合成相关基因。方法以华细辛地下部分(根)和地上部分(叶片)2个样本作为供试材料,采用Illumina Hiseq 4000进行转录组测序,并从头拼接,对Unigene进行功能注释和差异性分析。结果共获得12.25 Gb数据,拼接得到129 003个Unigene,可归类于52个GO分类,涉及363条KEGG代谢通路。分析发现,共有439个差异表达基因,其中上调基因占38.3%,下调基因占61.7%,差异较大;136个Unigene与苯丙素类次生代谢途径相关,其中44个Unigene参与甲基丁香酚的生物合成过程。结论本研究得到大量华细辛转录本信息,所发掘的与甲基丁香酚生物合成相关的Unigene为相关基因的分离、功能验证及其表达调控机制的阐明提供了重要依据。Objective To obtain the transcriptome sequence database and differentially expressed genes of Asarum sieboldii, and to identify the genes related to the biosynthesis of methyleugenol, the main chemical compound in this species. Methods Roots and leaves of the plants were chosen as experiment materials. The transcriptome sequence database was constructed by applying an Illumina Hiseq 4000 Sequencing Platform. Unigenes were assembled by BLAST similarity searches and annotated with GO and KEGG orthologs identifiers. Moreover, differentially expressed genes were analyzed. Results 12.25 Gb database was obtained, among which 129 003 unigenes were annotated to be involved in 52 GO-terms and 363 metabolic pathways. After analysis, 439 differentially expressed genes were observed, the up-regulated genes account for 38.3% and the down-regulated genes account for 61.7%. In addition, 136 unigenes involved in phenylpropanoid biosynthesis in A. sieboldii, and 44 unigenes that were associated with biosynthesis of methyleugenol were identified. Conclusion Unigenes explored in this study will significantly contribute to genome-wide research and analysis of this species.

关 键 词：华细辛 转录组 苯丙素类生物合成途径 甲基丁香酚 UNIGENE 

分 类 号：R282.12[医药卫生—中药学]