电离辐射对大鼠颌下腺钙离子激活型钾通道的影响  

Effects of Irradiation on IK1 and BK Channels in Rat Submandibular Glands.

作　　者：朱红华[1,2] 公柏娟[1] 宋子琦 鞠昊[1] 冯婧[1] 李志民[1] 孙宏晨[3] 

机构地区：[1]吉林大学口腔医院放射科,吉林长春130021 [2]桂林医学院附属医院口腔科,广西桂林541001 [3]吉林大学口腔医学院牙发育及颌骨重塑吉林省重点实验室,吉林长春130021

出　　处：《口腔医学研究》2017年第8期828-831,共4页Journal of Oral Science Research

基　　金：吉林省科技发展计划项目(编号:20170414047GH);吉林省教育厅"十三五"科学技术项目(编号:JJKH20170872KJ)

摘　　要：目的:通过研究电离辐射对大鼠颌下腺腺泡细胞膜上BK通道和IK通道开放的影响,探讨照射后唾液腺分泌功能降低的可能分子机制。方法:将大鼠随机分为对照组和照射组,对照射组大鼠的颌下腺区一次性15Gy X线照射,对照组不照射。分别在照射后3d、15d和40d取出大鼠颌下腺,胶原蛋白酶消化,从每只大鼠颌下腺消化液中挑选1个状态好的细胞,全细胞膜片钳技术研究大鼠颌下腺腺泡细胞膜上BK和IK1通道电流的变化。结果:1)在+80mV时,相比对照组大鼠颌下腺腺泡细胞膜上BK通道电流密度,照射后3d没有显著性差异,照射后15d减少9.7%(P<0.05),照射后40d减少13.8%(P<0.01)。2)在+80mV时,相比对照组大鼠颌下腺腺泡细胞膜上IK1通道电流密度,照射后3d没有显著性差异,照射后15d减少12.8%(P<0.05),照射后40d减少17.1%(P<0.01)。结论:电离辐射所致的大鼠唾液腺分泌功能减退可能与辐射后大鼠颌下腺腺泡细胞膜上BK和IK1通道开放电流减少导致的K+外流减少有关。Objective：To explore the mechanism of irradiation-induced salivary glands hypofunction by investigating the change of IK1 and BK channels electric current after irradiation in rat submandibular gland.Methods：Male Wistar rats,weight 180-220 g,were randomly divided into control group and irradiation group.The bilaterally submandibular glands of irradiation group were irradiated with a single dose of 15 Gy.The control rats were only anesthesia but no irradiated.On the pre-IR,post-IR 3rd day,15 th day and 40 th day,respectively,the macroscopic K＋currents change of IK1 and BK channels in acinar cells of submandibular glands were recorded with whole-cell patch clamp experiments.Results：At＋80mV,in compared with control group,the current density of BK channel and IK1 channel of 3day group was not significant different,and 15 day group reduced 9.7% and 12.8%,respectively（P〈0.05）,and 40 day group reduced 13.8%and 17.1%,respectively（P〈0.01）.Conclusion：Irradiation can reduce the K^＋current densities of BK channel and IK1 channel in rat submandibular gland,which may be one reason for radiation-induced salivary glands hypofunction.

关 键 词：口干 钾通道 电离辐射 颌下腺 膜片钳 

分 类 号：R730.55[医药卫生—肿瘤]